Positive Selection of V Beta 8+ CD4-8- Thymocytes by Class I Molecules Expressed by Hematopoietic Cells

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1993 Sep 1

PMID 8350060

Citations 52

Authors

M Bix

M Coles

D Raulet

Affiliations

Soon will be listed here.

Abstract

A small subset of T cells of mature phenotype express the alpha/beta T cell receptor, but not CD4 and CD8 coreceptors (alpha/beta double-negative [DN] cells). The repertoire of V beta usage of alpha/beta DN cells is strongly biased towards V beta 8 expression, suggesting that the formation of the population is subject to selection. We now report that deficiency of class I expression leads to a strongly depressed frequency of V beta 8+ DN cells, but has little effect on V beta 8- DN cells. Studies of hematopoietic chimeras between class I+ and class I- mice demonstrated that expression of class I molecules by hematopoietic cells is necessary and sufficient for selection of most V beta 8 DN cells. The lack of a role for class I expression by thymic epithelial cells suggests that the mechanism of selection of these cells by class I differs significantly from the mechanism of selection of conventional T cells. Models to explain the selection of these cells as well as their possible function in vivo are discussed.

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