Bacteriophage Mu DNA Replication is Stimulated by Non-essential Early Functions

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1982 Jan 1

PMID 7050622

Citations 12

Authors

T Goosen

M Giphart-Gassler

P Van de Putte

Affiliations

Soon will be listed here.

Abstract

The replication of a spontaneous Kil- mutant of bacteriophage Mu has been investigated. The Kil- mutation (Mucts62-13/4), which was introduced into a defective prophage, is pleiotrophic, leading to the loss of also the Gam, Cim and Sot functions. The mutation is caused by an insertion with the characteristics of IS1, located just outside the B gene. Mucts62-1 3/4 phages form extremely small plaques on wildtype indicator strains. The replication of the insertion mutant as compared to Mucts62 is strongly reduced. Normal replication could be restored by relieving the polarity of the insertion or by complementation with defective prophages which express all early functions. Apparently, early genes other than A and B are involved in normal Mu DNA replication.

Citing Articles

Twenty Years of Collaboration to Sort out Phage Mu Replication and Its Dependence on the Mu Central Gyrase Binding Site.

Toussaint A, Higgins N Viruses. 2023; 15(3).

PMID: 36992345 PMC: 10052514. DOI: 10.3390/v15030637.

The Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku.

dAdda di Fagagna F, Weller G, Doherty A, Jackson S EMBO Rep. 2003; 4(1):47-52.

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Neighboring plasmid sequences can affect Mini-Mu DNA transposition in the absence of expression of the bacteriophage Mu semi-essential early region.

Harel J, DuBow M Arch Microbiol. 1994; 161(5):418-24.

PMID: 8042905 DOI: 10.1007/BF00288953.

Mu DNA replication in vitro: criteria for initiation.

Higgins N, Olivera B Mol Gen Genet. 1984; 194(1-2):60-4.

PMID: 6328220 DOI: 10.1007/BF00383498.

Mini-Mu-duction as a test for genetic complementation in Escherichia coli.

Cronan Jr J J Bacteriol. 1984; 158(1):357-61.

PMID: 6232261 PMC: 215425. DOI: 10.1128/jb.158.1.357-361.1984.

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