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Two Classes of Mu Lig Mutants: the Thermosensitives for Integration and Replication and the Hyperproducers for Ligase

Overview
Journal Nucleic Acids Res
Publisher Oxford University Press
Specialty Biochemistry
Date 1980 Dec 11
PMID 7008031
Citations 5
Authors
L Paolozzi
P Ghelardini
J C Liebart
A Capozzoni
C MARCHELLI
Affiliations
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Abstract

We have previously shown that Mu restores an active DNA replication at non-permissive temperature in E. coli K12 ligts7 strains. In this paper we describe two new types of phage mutants for the Mu lig function. The Mu ligts mutants are conditional lethals defective for both integration and replication of DNA, unable to 'complement' the bacterial lig mutation; the map between B and lys. The mutants of the other type, on the other hand, are able to restore to a maximum level the activity impaired by the ligts7 mutation in the host. We suggest the hypothesis that the product of Mu lig gene could be part of a complex as a topoisomerase.

Citing Articles

The Escherichia coli histone-like protein HU affects DNA initiation, chromosome partitioning via MukB, and cell division via MinCDE.

Jaffe A, Vinella D, Dari R J Bacteriol. 1997; 179(11):3494-9.

PMID: 9171392 PMC: 179140. DOI: 10.1128/jb.179.11.3494-3499.1997.

Bacteriophage Mu DNA replication is stimulated by non-essential early functions.

Goosen T, Giphart-Gassler M, Van de Putte P Mol Gen Genet. 1982; 186(1):135-9.

PMID: 7050622 DOI: 10.1007/BF00422925.

Escherichia coli K-12 gyrB gene product is involved in the lethal effect of the ligts2 mutant of bacteriophage Mu.

Ghelardini P, Liebart J, MARCHELLI C, Pedrini A, Paolozzi L J Bacteriol. 1984; 157(2):665-8.

PMID: 6319373 PMC: 215300. DOI: 10.1128/jb.157.2.665-668.1984.

Suppression of the thermosensitive DNA ligase mutations in Escherichia coli K12 through modulation of gene expression induced by phage Mu.

Ghelardini P, Liebart J, Paolozzi L, Pedrini A Mol Gen Genet. 1989; 216(1):31-6.

PMID: 2543906 DOI: 10.1007/BF00332227.

Synchronous division induced in Escherichia coli K12 by gemts mutants of phage Mu.

Paolozzi L, Nicosia A, Liebart J, Ghelardini P Mol Gen Genet. 1989; 218(1):13-7.

PMID: 2528678 DOI: 10.1007/BF00330559.

References
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