Mu-1 Directed Inhibition of DNA Breakdown in Escherichia Coli, RecA Cells

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1978 Aug 4

PMID 360037

Citations 14

Authors

F Van Vliet

M Couturier

J de Lafonteyne

E Jedlicki

Affiliations

Soon will be listed here.

Abstract

The spontaneous DNA breakdown exhibited by recA strains, is reduced after heat induction of a thermoinducible Mu-1 prophage. This inhibition is dependent upon RNA synthesis, suggesting that Mu-1 directs synthesis of a recBC nuclease inhibitor, analogous to the product of the lambda gam gene. The genetic evidence presented here shows that Mu-1 enables a lambda red gam phage to grow on a recA host. The in vitro assay for ATP-dependent exonuclease activity reveals a complete inhibition of this activity 30 min after induction of the Mu-1 prophage.

Citing Articles

Phage Mu Gam protein promotes NHEJ in concert with ligase.

Bhattacharyya S, Soniat M, Walker D, Jang S, Finkelstein I, Harshey R Proc Natl Acad Sci U S A. 2018; 115(50):E11614-E11622.

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Bacteriophage Mu DNA replication is stimulated by non-essential early functions.

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Partial purification and properties of an exonuclease inhibitor induced by bacteriophage Mu-1.

G Williams J, Radding C J Virol. 1981; 39(2):548-58.

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Plasmid control of recombination of E. coli K12.

Chernin L, Ovadis M Mol Gen Genet. 1980; 179(2):399-407.

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Transduction of multi-copy plasmid pBR322 by bacteriophage Mu.

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References

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