Generation of Functional Human T Cell Hybrids

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1981 Dec 1

PMID 6976408

Citations 8

Authors

O Irigoyen

P V Rizzolo

Y Thomas

L Rogozinski

L Chess

Affiliations

Soon will be listed here.

Abstract

Human T cell hybrids were generated by fusing lectin-activated normal and leukemic human T cells with an aminopterin-sensitive human T cell line. This mutant cell line, designated CEM-T15, was derived from the human T cell line CEM after chemical mutagenesis with ethane methylsulfonate and subsequent culture in medium containing 6-thioguanine. After polyethylene glycol-induced fusion, the cells were cultured in hypoxanthine-aminopterin-thymidine selective medium. More than 5 wk after fusion, evidence for successful hybridization was obtained by three independent criteria: (a) The majority of the cultures contained cells expressing the OKT3 surface antigen: this antigen is expressed on normal T cells but not on CEM-T15 cells. (b) Most of the cultures contained polyploid cells. (c) Some of the cultures provided helper activity in the generation of antibody-forming cells. This functional activity is absent from the CEM-T15 parental cell line. Evidence for functional stability of the hybrids greater than 20 wk after fusion was provided by several clones that not only continue growing exponentially but also maintain expression of OKT3 surface antigen and high levels of helper function. These T cell hybrids constructed using antigen-specific human T cells should be of considerable importance in further studies of the immunobiology of human T cells.

Citing Articles

Production of functional human T-T hybridomas in selection medium lacking aminopterin and thymidine.

Foung S, Sasaki D, Grumet F, Engleman E Proc Natl Acad Sci U S A. 1982; 79(23):7484-8.

PMID: 6984190 PMC: 347364. DOI: 10.1073/pnas.79.23.7484.

Human T cell hybridomas secreting factors for IgA-specific help, polyclonal B cell activation, and B cell proliferation.

Mayer L, Fu S, KUNKEL H J Exp Med. 1982; 156(6):1860-5.

PMID: 6983562 PMC: 2186861. DOI: 10.1084/jem.156.6.1860.

Antigen-specific human T-cell hybridomas with helper activity.

DeFreitas E, Vella S, Linnenbach A, Zmijewski C, KOPROWSKI H, Croce C Proc Natl Acad Sci U S A. 1982; 79(21):6646-50.

PMID: 6983073 PMC: 347185. DOI: 10.1073/pnas.79.21.6646.

Human T cell hybridomas secreting immune interferon.

Le J, Vilcek J, Saxinger C, Prensky W Proc Natl Acad Sci U S A. 1982; 79(24):7857-61.

PMID: 6818554 PMC: 347448. DOI: 10.1073/pnas.79.24.7857.

Characterization of a human X mouse T cell hybridoma and identification of a clone secreting and binding interleukin-2.

Durrant L, Parkar M, Kenworthy N, Taylor G Immunology. 1984; 52(1):117-28.

PMID: 6609121 PMC: 1454574.

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