Concanavalin A-inducible, Interleukin-2-producing T Cell Hybridoma

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1980 Oct 1

PMID 6968339

Citations 51

Authors

L Harwell

B Skidmore

P Marrack

J Kappler

Affiliations

Soon will be listed here.

Abstract

The fusion of an AKR T cell tumor line to normal B6D2F1, T cells resulted in the production of a cloned T cell hybridoma (FS6-14.13) inducible with the mitogen concanavalin A (Con A). The supernate from Con A-stimulated hybridoma cells was active both in the stimulation of an anti-sheep red blood cell response by partially T cell-depleted B cells and in the stimulation of the growth of antigen-specific T cell blasts. The active principle in both assays had a molecular weight of approximately 30-40,000. These results indicated the presence of interleukin 2 (IL2) in the hybridoma supernate. The activity of the hybridoma supernate in B cell responses was dependent on the presence of adherent cells and a few contaminating T cells. On the other hand, Con A-stimulated supernates from normal spleen cells were active after either adherent cell removal or severe T cell depletion. These results suggested that IL2 was the only active helper factor in the hybridoma supernate, but that additional helper factors were present in supernates from Con A-stimulated normal spleen cells.

Citing Articles

Gamma delta T-cell lines isolated from intestinal epithelium respond to a B-cell lymphoma.

Sano Y, Dudley E, Carding S, Lin R, Hayday A, Janeway Jr C Immunology. 1993; 80(3):388-94.

PMID: 8288315 PMC: 1422228.

T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells.

Isakson P, Pure E, Vitetta E, Krammer P J Exp Med. 1982; 155(3):734-48.

PMID: 7038025 PMC: 2186625. DOI: 10.1084/jem.155.3.734.

T cell replacing factor substitutes for an I-J+ idiotype-specific T helper cell.

Jayaraman S, Swierkosz J, Bellone C J Exp Med. 1982; 155(2):641-6.

PMID: 7035601 PMC: 2186601. DOI: 10.1084/jem.155.2.641.

Fever and immunoregulation: hyperthermia, interleukins 1 and 2, and T-cell proliferation.

Duff G, Durum S Yale J Biol Med. 1982; 55(5-6):437-42.

PMID: 6985107 PMC: 2596539.

B-cell growth factor: distinction from T-cell growth factor and B-cell maturation factor.

Leanderson T, Lundgren E, Ruuth E, Borg H, Persson H, Coutinho A Proc Natl Acad Sci U S A. 1982; 79(23):7455-9.

PMID: 6984188 PMC: 347358. DOI: 10.1073/pnas.79.23.7455.

References

Mishell R, Dutton R . Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967; 126(3):423-42. PMC: 2138377. DOI: 10.1084/jem.126.3.423. View

Katz D, Osborne Jr D . The allogeneic effect in inbred mice. II. Establishment of the cellular interactions required for enhancement of antibody production by the graft-versus-host reaction. J Exp Med. 1972; 136(3):455-65. PMC: 2139261. DOI: 10.1084/jem.136.3.455. View

Kappler J . A micro-technique for hemolytic plaque assays. J Immunol. 1974; 112(3):1271-4. View

Waldmann H, Munro A . T cell-dependent mediator in the immune response. II. Physical and biological properties. Immunology. 1974; 27(1):53-64. PMC: 1445584. View

Taussig M, Munro A, Campbell R, David C, Staines N . Antigen-specific T-cell factor in cell cooperation. Mapping within the I region of the H-2 complex and ability to cooperate across allogeneic barriers. J Exp Med. 1975; 142(3):694-700. PMC: 2189912. DOI: 10.1084/jem.142.3.694. View

Marrack P, Kappler J . Antigen-specific and nonspecific mediatiors of T cell/B cell cooperation. II. Two helper T cells distinguished by their antigen sensitivities. J Immunol. 1976; 116(5):1373-8. View

Harwell L, Kappler J, Marrack P . Antigen-specific and nonspecific mediators of T cell/B cell cooperation. III. Characterization of the nonspecific mediator(s) from different sources. J Immunol. 1976; 116(5):1379-84. View

Bergman Y, Haimovich J . Characterization of a carcinogen-induced murine B lymphocyte cell line of C3H/eB origin. Eur J Immunol. 1977; 7(7):413-7. DOI: 10.1002/eji.1830070702. View

Kappler J, Marrack P . The role of H-2-linked genes in helper T-cell function. I. In vitro expression in B cells of immune response genes controlling helper T-cell activity. J Exp Med. 1977; 146(6):1748-64. PMC: 2181905. DOI: 10.1084/jem.146.6.1748. View

10.

Kontiainen S, Simpson E, Bohrer E, Beverley P, Herzenberg L, Fitzpatrick W . T-cell lines producing antigen-specific suppressor factor. Nature. 1978; 274(5670):477-80. DOI: 10.1038/274477a0. View

11.

Skidmore B, Miller L . The determination of lymphoid cell chimerism using peripheral blood lymphocytes from murine bone marrow chimeras. J Immunol Methods. 1978; 24(3-4):337-43. DOI: 10.1016/0022-1759(78)90136-9. View

12.

Hoffmann M, Watson J . Helper T cell-replacing factors secreted by thymus-derived cells and macrophages: cellular requirements for B cell activation and synergistic properties. J Immunol. 1979; 122(4):1371-5. View

13.

Watson J, Aarden L, Shaw J, Paetkau V . Molecular and quantitative analysis of helper T cell-replacing factors on the induction of antigen-sensitive B and T lymphocytes. J Immunol. 1979; 122(5):1633-8. View

14.

Schrier R, Skidmore B, Kurnick J, Goldstine S, Chiller J . Propagation of antigen-specific T cell helper function in vitro. J Immunol. 1979; 123(6):2525-31. View

15.

Swain S, Dutton R . Production of Con A-induced helper T cell replacing factor requires a T cell and an Ia-positive non-T cells. J Immunol. 1980; 124(1):437-44. View

16.

Watson J, Gillis S, Marbrook J, Mochizuki D, Smith K . Biochemical and biological characterization of lymphocyte regulatory molecules. I. Purification of a class of murine lymphokines. J Exp Med. 1979; 150(4):849-61. PMC: 2185677. DOI: 10.1084/jem.150.4.849. View

17.

Smith K, Gillis S, Baker P, McKenzie D, Ruscetti F . T-cell growth factor-mediated T-cell proliferation. Ann N Y Acad Sci. 1979; 332:423-32. DOI: 10.1111/j.1749-6632.1979.tb47136.x. View

18.

Keller D, Swierkosz J, Marrack P, Kappler J . Two types of functionally distinct, synergizing helper T cells. J Immunol. 1980; 124(3):1350-9. View

19.

Gillis S, Smith K, Watson J . Biochemical characterization of lymphocyte regulatory molecules. II. Purification of a class of rat and human lymphokines. J Immunol. 1980; 124(4):1954-62. View

20.

McDougal J, Gordon D . Generation of T-helper cells in vitro. II. Analysis of supernates derived from T-helper cell cultures. J Exp Med. 1977; 145(3):693-708. PMC: 2180701. DOI: 10.1084/jem.145.3.693. View