Alteration of Clonal Profile. III. T15 Ontogenetic Advantages Are Not Sufficient for Establishing Idiotypic Dominance in Adoptive Transfer

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1981 Nov 1

PMID 6975348

Citations 1

Authors

Z S Quan

J Quintans

Affiliations

Soon will be listed here.

Abstract

We have examined the ontogeny of BALB/c plaque-forming cell (PFC) responses to phosphorylcholine (PC) from fetal and neonatal liver by using the (CBA/N x BALB/c)F1 transplantation model. In this system, thymus-dependent (PC-keyhole limpet hemocyanin) and thymus-independent class 1 (PC-Brucella abortus, PC-lipopolysaccharide) PC antigens stimulate B cell subpopulations, which functionally emerge early after transfer. Responsiveness to a thymus-independent class 2 antigen, C-polysaccharide extract of a Streptococcus pneumoniae mutant, is acquired later. The response to PC antigens tested initially exhibited T15 dominance. Non-T15 clones, which are not expressed to a great degree in normal BALB/c mice, are inherently slow in their rate of maturation; in adoptive transfer, however, they eventually comprise much of the transplanted anti-PC PFC response. Obviously, the advantages the T15 subset has in ontogeny do not result in idiotypic dominance once the immature cells are removed from the intact BALB/c environment. We discuss possible regulatory mechanisms involved in the alteration of the T15+:T15- ratio.

Citing Articles

Mice with the xid defect have helper cells for T15 idiotype-dominant anti-phosphorylcholine primary and secondary plaque-forming cells responses.

Quintans J, Quan Z, Arias M J Exp Med. 1982; 155(4):1245-50.

PMID: 7038026 PMC: 2186659. DOI: 10.1084/jem.155.4.1245.

References

Andersson B, Blomgren H . T-cell response to polyvinyl pyrrolidone is linked to maturity of B cells. Nature. 1975; 253(5491):476-7. DOI: 10.1038/253476a0. View

Anderson H, McCarty M . The occurrence in the rabbit of an acute phase protein analogous to human C reactive protein. J Exp Med. 1951; 93(1):25-36. PMC: 2136052. DOI: 10.1084/jem.93.1.25. View

INMAN J . Thymus-independent antigens: the preparation of covalent, hapten-ficoll conjugates. J Immunol. 1975; 114(2 Pt 1):704-9. View

Quintans J, Cosenza H . Antibody response to phosphorylcholine in vitro. II. Analysis of T-dependent and T-independent responses. Eur J Immunol. 1976; 6(6):399-405. DOI: 10.1002/eji.1830060605. View

Gold E, Fudenberg H . Chromic chloride: a coupling reagent for passive hemagglutination reactions. J Immunol. 1967; 99(5):859-66. View

Cunningham A, SZENBERG A . Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968; 14(4):599-600. PMC: 1409394. View

RITTENBERG M, PRATT K . Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969; 132(2):575-81. DOI: 10.3181/00379727-132-34264. View

Chesebro B, Metzger H . Affinity labeling of a phosphorylcholine binding mouse myeloma protein. Biochemistry. 1972; 11(5):766-71. DOI: 10.1021/bi00755a014. View

Cosenza H, Kohler H . Specific inhibition of plaque formation to phosphorylcholine by antibody against antibody. Science. 1972; 176(4038):1027-9. DOI: 10.1126/science.176.4038.1027. View

10.

Cosenza H, Kohler H . Specific suppression of the antibody response by antibodies to receptors. Proc Natl Acad Sci U S A. 1972; 69(9):2701-5. PMC: 427020. DOI: 10.1073/pnas.69.9.2701. View

11.

Sher A, Cohn M . Inheritance of an idiotype associated with the immune response of inbred mice to phosphorylcholine. Eur J Immunol. 1972; 2(4):319-26. DOI: 10.1002/eji.1830020405. View

12.

CLAFLIN J . Uniformity in the clonal repertoire for the immune response to phosphorylcholine in mice. Eur J Immunol. 1976; 6(10):669-74. DOI: 10.1002/eji.1830061002. View

13.

LEWIS G, Goodman J . Carrier-directed anti-hapten responses by B-cell subsets. J Exp Med. 1977; 146(1):1-10. PMC: 2180734. DOI: 10.1084/jem.146.1.1. View

14.

Mond J, Lieberman R, INMAN J, Mosier D, Paul W . Inability of mice with a defect in B-lymphocyte maturation to respond to phosphorycholine on immunogenic carriers. J Exp Med. 1977; 146(4):1138-42. PMC: 2180827. DOI: 10.1084/jem.146.4.1138. View

15.

Kaplan D, Quintans J, Kohler H . Clonal dominance: loss and restoration in adoptive transfer. Proc Natl Acad Sci U S A. 1978; 75(4):1967-70. PMC: 392463. DOI: 10.1073/pnas.75.4.1967. View

16.

Quintans J, Kaplan R . Failure of CBA/N mice to respond to thymus-dependent and thymus-independent phosphorylcholine antigens. Cell Immunol. 1978; 38(2):294-301. DOI: 10.1016/0008-8749(78)90060-6. View

17.

Kaplan D, Quintans J . Alteration of clonal profile. I. Effect of sublethal irradiation on the responses to phosphorylcholine in BALB/c mice. J Exp Med. 1978; 148(4):987-95. PMC: 2185030. DOI: 10.1084/jem.148.4.987. View

18.

Quintans J, McKearn J, KAPLAN D . B cell heterogeneity. I. A study of B cell subpopulations involved in the reconstitution of an X-linked immune defect of B cell differentiation. J Immunol. 1979; 122(5):1750-6. View

19.

Forni L, Cazenave P, Cosenza H, Forsbeck K, Coutinho A . Expression of V-region-like determinants on Ig-negative precursors in murine fetal liver and bone marrow. Nature. 1979; 280(5719):241-3. DOI: 10.1038/280241a0. View

20.

Bottomly K, Mosier D . Mice whose B cells cannot produce the T15 idiotype also lack an antigen-specific helper T cell required for T15 expression. J Exp Med. 1979; 150(6):1399-409. PMC: 2185721. DOI: 10.1084/jem.150.6.1399. View