Isolation and Partial Characterization of Bacillus Subtilis Mutants Impaired in DNA Entry

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Apr 1

PMID 6801021

Citations 16

Authors

J A Mulder

G Venema

Affiliations

Soon will be listed here.

Abstract

Transformation-deficient mutants of Bacillus subtilis have been identified either by screening for a nuclease-deficient phenotype on methyl green-DNA agar or for nontransformability on transforming DNA-containing agar. After purification of the mutations causing a reduction in the entry of DNA, a set of isogenic entry-deficient strains was obtained. In addition to being entry deficient to various extents, the strains usually were less capable of association with DNA than the entry-proficient parent. Likewise, the specific transforming activity in the purified mutant strains continued to be less than that in the wild type. With the possible exception of one strain, no evidence was obtained that the mutant strains were impaired in recombination. Since the breakdown of transforming DNA to acid-soluble products correlated fairly well with the residual capacity of the strains to take up DNA, nucleolytic activity is likely to be involved in the entry of DNA in B. subtilis.

Citing Articles

From isotopically labeled DNA to fluorescently labeled dynamic pili: building a mechanistic model of DNA transport to the cytoplasmic membrane.

Zuke J, Burton B Microbiol Mol Biol Rev. 2024; 88(1):e0012523.

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The C terminus of the AddA subunit of the Bacillus subtilis ATP-dependent DNase is required for the ATP-dependent exonuclease activity but not for the helicase activity.

Haijema B, Venema G, Kooistra J J Bacteriol. 1996; 178(17):5086-91.

PMID: 8752323 PMC: 178302. DOI: 10.1128/jb.178.17.5086-5091.1996.

Bacterial gene transfer by natural genetic transformation in the environment.

Lorenz M, Wackernagel W Microbiol Rev. 1994; 58(3):563-602.

PMID: 7968924 PMC: 372978. DOI: 10.1128/mr.58.3.563-602.1994.

Translocation of DNA across bacterial membranes.

Dreiseikelmann B Microbiol Rev. 1994; 58(3):293-316.

PMID: 7968916 PMC: 372970. DOI: 10.1128/mr.58.3.293-316.1994.

Transformation-deficient mutants of Bacillus subtilis impaired in competence-specific nuclease activities.

Mulder J, Venema G J Bacteriol. 1982; 152(1):166-74.

PMID: 6811548 PMC: 221388. DOI: 10.1128/jb.152.1.166-174.1982.

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