Characterization of DNA Uptake by the Cyanobacterium Anacystis Nidulans

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1986 Aug 1

PMID 3093820

Citations 6

Authors

H Daniell

B A McFadden

Affiliations

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Abstract

The binding and uptake of nick-translated 32P-labeled pBR322 by Anacystis nidulans 6301 have been characterized. Both processes were considerably enhanced in permeaplasts compared to cells. The breakdown of labeled DNA was not correlated with binding or uptake by permeaplasts or cells. Uptake of DNA by permeaplasts was unaffected by: Mg2+ or Ca2+, light, or inhibitors of photophosphorylation such as valinomycin or gramicidin D in the presence or absence of NH4Cl. ATP at 2.5-10 mM inhibited both binding and uptake of labeled DNA by permeaplasts of A. nidulans whereas the ATP analog adenyl-5-yl imido-diphosphate was non-inhibitory in the same concentration range. In contrast to transformation of A. nidulans 6301 cells to ampicillin-resistance by pBR322, transformation to kanamycin-resistance by the plasmid pHUB4 was considerably enhanced in the dark. The transformation efficiency for permeaplasts by the plasmid pCH1 was 59% and 8% in the dark and light, respectively, whereas transformation of permeaplasts by pBR322 at an efficiency of 16% was absolutely light-dependent.

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References

Smith H, Wiersma K, Bron S, Venema G . Transformation in Bacillus subtilis: purification and partial characterization of a membrane-bound DNA-binding protein. J Bacteriol. 1983; 156(1):101-8. PMC: 215056. DOI: 10.1128/jb.156.1.101-108.1983. View

Mulder J, Venema G . Isolation and partial characterization of Bacillus subtilis mutants impaired in DNA entry. J Bacteriol. 1982; 150(1):260-8. PMC: 220108. DOI: 10.1128/jb.150.1.260-268.1982. View

Lacks S, Greenberg B . Single-strand breakage on binding of DNA to cells in the genetic transformation of Diplococcus pneumoniae. J Mol Biol. 1976; 101(2):255-75. DOI: 10.1016/0022-2836(76)90376-4. View

McFadden B, Torres-Ruiz J, Daniell H, Sarojini G . Interaction, functional relations and evolution of large and small subunits in Rubisco from prokaryota and eukaryota. Philos Trans R Soc Lond B Biol Sci. 1986; 313(1162):347-58. DOI: 10.1098/rstb.1986.0042. View

Kolowsky K, G Williams J, Szalay A . Length of foreign DNA in chimeric plasmids determines the efficiency of its integration into the chromosome of the cyanobacterium Synechococcus R2. Gene. 1984; 27(3):289-99. DOI: 10.1016/0378-1119(84)90073-8. View

Buitenwerf J, Venema G . Transformation in bacillus subtilis: fate of transforming DNA in transformation deficient mutants. Mol Gen Genet. 1977; 151(2):203-13. DOI: 10.1007/BF00338696. View

Smith H, Wiersma K, Venema G, Bron S . Transformation in Bacillus subtilis: a 75,000-dalton protein complex is involved in binding and entry of donor DNA. J Bacteriol. 1984; 157(3):733-8. PMC: 215319. DOI: 10.1128/jb.157.3.733-738.1984. View

Joenje H, Venema G . Different nuclease activities in competent and noncompetent Bacillus subtilis. J Bacteriol. 1975; 122(1):25-33. PMC: 235634. DOI: 10.1128/jb.122.1.25-33.1975. View

Sherman L, Van de Putte P . Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans. J Bacteriol. 1982; 150(1):410-3. PMC: 220131. DOI: 10.1128/jb.150.1.410-413.1982. View

10.

Tomaszek Jr T, Schuster S . Hydrolysis of adenyl-5-yl imidodiphosphate by beef heart mitochondrial ATPase. J Biol Chem. 1986; 261(5):2264-9. View

11.

van Nieuwenhoven M, Hellingwerf K, Venema G, Konings W . Role of proton motive force in genetic transformation of Bacillus subtilis. J Bacteriol. 1982; 151(2):771-6. PMC: 220324. DOI: 10.1128/jb.151.2.771-776.1982. View

12.

Daniell H, Sarojini G, McFadden B . Transformation of the cyanobacterium Anacystis nidulans 6301 with the Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1986; 83(8):2546-50. PMC: 323335. DOI: 10.1073/pnas.83.8.2546. View

13.

Golden S, Sherman L . A hybrid plasmid is a stable cloning vector for the cyanobacterium Anacystis nidulans R2. J Bacteriol. 1983; 155(3):966-72. PMC: 217787. DOI: 10.1128/jb.155.3.966-972.1983. View

14.

Kuhlemeier C, Borrias W, van den Hondel C, VAN ARKEL G . Vectors for cloning in cyanobacteria: construction and characterization of two recombinant plasmids capable of transformation of Escherichia coli K12 and Anacystis nidulans R2. Mol Gen Genet. 1981; 184(2):249-54. DOI: 10.1007/BF00272912. View

15.

Lurquin P, BEHKI R . Uptake of bacterial DNA by Chlamydomonas reinhardi. Mutat Res. 1975; 29(1):35-51. DOI: 10.1016/0027-5107(75)90019-6. View

16.

G Williams J, Szalay A . Stable integration of foreign DNA into the chromosome of the cyanobacterium Synechococcus R2. Gene. 1983; 24(1):37-51. DOI: 10.1016/0378-1119(83)90129-4. View

17.

Chauvat F, Astier C, Vedel F, Joset-Espardellier F . Transformation in the cyanobacterium Synechococcus R2: improvement of efficiency; role of the pUH24 plasmid. Mol Gen Genet. 1983; 191(1):39-45. DOI: 10.1007/BF00330887. View

18.

van den Hondel C, Keegstra W, Borrias W, VAN ARKEL G . Homology of plasmids in strains of unicellular Cyanobacteria. Plasmid. 1979; 2(3):323-33. DOI: 10.1016/0147-619x(79)90016-7. View

19.

van den Hondel C, Verbeek S, van der Ende A, Weisbeek P, Borrias W, VAN ARKEL G . Introduction of transposon Tn901 into a plasmid of Anacystis nidulans: preparation for cloning in cyanobacteria. Proc Natl Acad Sci U S A. 1980; 77(3):1570-4. PMC: 348538. DOI: 10.1073/pnas.77.3.1570. View

20.

Smith H, Wiersma K, Venema G, Bron S . Transformation in Bacillus subtilis: further characterization of a 75,000-dalton protein complex involved in binding and entry of donor DNA. J Bacteriol. 1985; 164(1):201-6. PMC: 214230. DOI: 10.1128/jb.164.1.201-206.1985. View