Eosinophilic Differentiation of the Human Promyelocytic Leukemia Cell Line, HL-60

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1984 Jul 1

PMID 6588134

Citations 21

Authors

S A Fischkoff

A Pollak

G J Gleich

J R Testa

S Misawa

T J Reber

Affiliations

Soon will be listed here.

Abstract

HL-60 promyelocytic leukemia cells differentiated to eosinophils and eosinophilic precursors when cultured under mildly alkaline conditions (pH 7.6-7.8) for 7 d without refeeding. New cytoplasmic granules appeared blue in the least mature cells and red in the most mature cells when stained with Wright-Giemsa. The granules also stained with Luxol-fast-blue, a characteristic of eosinophil granules. Furthermore, most cells contained the eosinophil major basic protein (MBP); the Charcot-Leyden Crystal (CLC) protein (lysophospholipase), eosinophil peroxidase, acid phosphatase, and arylsulfatase were also detected in a portion of these cells. The eosinophil major basic protein was found in a high proportion of undifferentiated cells, and thus may be constituitively produced. By examining finely banded chromosomes, translocation break points were demonstrated at q22 on one chromosome 16 and at q23 on the other homologue; abnormalities in this region of the long arm of 16 are a characteristic finding in the recently described syndrome of acute myelomonocytic leukemia (AMMoL) with abnormal bone marrow eosinophils. In common with the bone marrow eosinophils in these patients, the HL-60 eosinophil granules contained chloroacetate esterase and periodic-acid Schiff (PAS) reactive material; crystalloid inclusions were rare. Therefore, the HL-60 cell line appears to be an in vitro model for eosinophilopoiesis and may be specially suited for the study of the abnormal eosinophils seen in certain malignant conditions.

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References

Goldfischer S . The cytochemical demonstration of lysosomal aryl sulfatase activity by light and electron microscopy. J Histochem Cytochem. 1965; 13(6):520-3. DOI: 10.1177/13.6.520. View

LEDER L . [Acute myelo-monocytic leukemia with atypical naphthol-AS-D-chloracetate esterase positive eosinophils]. Acta Haematol. 1970; 44(1):52-62. DOI: 10.1159/000208662. View

Ceccarini C, Eagle H . pH as a determinant of cellular growth and contact inhibition. Proc Natl Acad Sci U S A. 1971; 68(1):229-33. PMC: 391201. DOI: 10.1073/pnas.68.1.229. View

Schaefer H, Hellriegel K, Hennekeuser H, Hubner G, Zach J, Fischer R . [Eosinophilic leukemia, an immature cell myelosis with chloroacetate esterase-positive eosinophilia. A morphological and cytochemical study on the problem of monophylic myeloses]. Blut. 1973; 26(1):7-19. DOI: 10.1007/BF01631306. View

PARMLEY R, SPICER S . Cytochemical and ultrastructural identification of a small type granule in human late eosinophils. Lab Invest. 1974; 30(5):557-67. View

Zucker-Franklin D, Grusky G . The identification of eosinophil colonies in soft-agar cultures by differential staining for peroxidase. J Histochem Cytochem. 1976; 24(12):1270-2. DOI: 10.1177/24.12.63511. View

Collins S, Ruscetti F, Gallagher R, Gallo R . Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc Natl Acad Sci U S A. 1978; 75(5):2458-62. PMC: 392573. DOI: 10.1073/pnas.75.5.2458. View

STAVEM P, Ly B, Blichfeldt P, Andreassen P . Acute eosinophilo-myelomonocytic leukaemia, one of the 'in between leukaemias'. Scand J Haematol. 1978; 21(4):355-9. DOI: 10.1111/j.1600-0609.1978.tb00377.x. View

Hyman P, Teichberg S, Starrett S, Vinciguerra V, DEGNAN T . Secretion of primary granules from developing human eosinophilic promyelocytes. Proc Soc Exp Biol Med. 1978; 159(3):380-5. DOI: 10.3181/00379727-159-40352. View

10.

PRESENTEY B, Jerushalmy Z, Mintz U . Eosinophilic leukaemia: morphological, cytochemical, and electron microscopic studies. J Clin Pathol. 1979; 32(3):261-71. PMC: 1145633. DOI: 10.1136/jcp.32.3.261. View

11.

Rovera G, Santoli D, Damsky C . Human promyelocytic leukemia cells in culture differentiate into macrophage-like cells when treated with a phorbol diester. Proc Natl Acad Sci U S A. 1979; 76(6):2779-83. PMC: 383692. DOI: 10.1073/pnas.76.6.2779. View

12.

Gallagher R, Collins S, Trujillo J, McCredie K, AHearn M, Tsai S . Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood. 1979; 54(3):713-33. View

13.

BREITMAN T, Collins S, Keene B . Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic cell line, HL-60. Exp Cell Res. 1980; 126(2):494-8. DOI: 10.1016/0014-4827(80)90296-7. View

14.

Laskin J, Mufson R, Weinstein I, Engelhardt D . Identification of a distinct phase during melanogenesis that is sensitive to extracellular pH and ionic strength. J Cell Physiol. 1980; 103(3):467-74. DOI: 10.1002/jcp.1041030312. View

15.

Johnson G, Metcalf D . Detection of a new type of mouse eosinophil colony by Luxol-fast-blue staining. Exp Hematol. 1980; 8(5):549-61. View

16.

Weller P, Goetzl E, Austen K . Identification of human eosinophil lysophospholipase as the constituent of Charcot-Leyden crystals. Proc Natl Acad Sci U S A. 1980; 77(12):7440-3. PMC: 350519. DOI: 10.1073/pnas.77.12.7440. View

17.

LOZZIO B, Lozzio C, Bamberger E, Feliu A . A multipotential leukemia cell line (K-562) of human origin. Proc Soc Exp Biol Med. 1981; 166(4):546-50. DOI: 10.3181/00379727-166-41106. View

18.

Zetterberg A, Engstrom W . Mitogenic effect of alkaline pH on quiescent, serum-starved cells. Proc Natl Acad Sci U S A. 1981; 78(7):4334-8. PMC: 319784. DOI: 10.1073/pnas.78.7.4334. View

19.

Tai P, Spry C . The mechanisms which produce vacuolated and degranulated eosinophils. Br J Haematol. 1981; 49(2):219-26. DOI: 10.1111/j.1365-2141.1981.tb07218.x. View

20.

Lu L, Broxmeyer H, Pelus L, Andreeff M, Moore M . Detection of luxol-fast-blue positive cells in human promyelocytic leukemia cell line HL-60. Exp Hematol. 1981; 9(9):887-92. View