Complete MRNA Coding Sequence of the Acetylcholine Binding Alpha-subunit of Torpedo Marmorata Acetylcholine Receptor: a Model for the Transmembrane Organization of the Polypeptide Chain

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 Apr 1

PMID 6572962

Citations 80

Authors

A Devillers-Thiery

J Giraudat

M Bentaboulet

J P Changeux

Affiliations

Soon will be listed here.

Abstract

A 1,350-base-pair-long cDNA clone, named p alpha-2, was isolated by hybridization to the previously characterized clone p alpha-1 and found to be specific for the alpha-subunit of the Torpedo marmorata acetylcholine receptor. The nucleotide sequences of both cDNA inserts were analyzed and the sequence of the complete coding region and part of the 5' and 3' untranslated regions of the alpha-chain mRNA was determined. The complete amino acid sequence of the alpha-chain precursor is presented and used to develop a model for the transmembrane organization of the polypeptide.

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