Residues 377-389 from the Delta Subunit of Torpedo Californica Acetylcholine Receptor Are Located in the Cytoplasmic Surface

Overview

Journal J Protein Chem

Specialties Biochemistry
Chemistry

Date 1994 Jan 1

PMID 8011073

Citations 1

Authors

A Iriarte

M Martinez-Carrion

Affiliations

Soon will be listed here.

Abstract

Torpedo californica acetylcholine receptor (AcChR) enriched, sealed vesicles have been specifically labeled on the cytoplasmic surface with pyridoxal 5'-phosphate (Perez-Ramirez, B., and Martinez-Carrion, M., 1989, Biochemistry 28, 5034-5040). After chromatography of the peptide fragments produced by trypin digestion of labeled AcChR, several fractions containing the phosphopyridoxyl label were obtained. Edman degradation identified one of the fractions, with sequence SRSELMFEKQSER, as corresponding to residues 377-389 in the delta subunit (primary structure). The latter must be a cytoplasmic region of this transmembranous protein, and residue delta K385 must reside in a water-soluble exposed domain of the cytosolic side of the membrane. Introduction of phosphopyridoxyl residues allows for their potential use as probes of conformational changes in the cytosolic surface of the receptor molecule.

Citing Articles

Mapping of exposed surfaces of the nicotinic acetylcholine receptor by identification of iodinated tyrosine residues.

Mund M, Weise C, Franke P, Hucho F J Protein Chem. 1997; 16(3):161-70.

PMID: 9155087 DOI: 10.1023/a:1026399123574.

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