The Nucleotide Sequence of the Replication Control Region of the Resistance Plasmid R1drd-19

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1981 Jan 1

PMID 6261081

Citations 29

Authors

P Stougaard

S Molin

K Nordstrom

F G Hansen

Affiliations

Soon will be listed here.

Abstract

The region of plasmid R 1 containing the replication control genes has been sequenced using the Maxam-Gilbert method. The nucleotide sequence of two small PstI restriction fragments (a total of about 1,000 base pairs) was determined for the wild-type R 1 plasmid as well as for two different copy mutants. It was found that one copy mutant has a single base substitution in the fragment which was recently shown to harbor an important inc/cop gene (Molin and Nordström 1980). Furthermore, the sequence indicates the presence of a structural gene that codes for a polypeptide of size 10,500 daltons. Possible gene products predicted from the nucleotide sequences and their role in replication control are discussed.

Citing Articles

Convergent transcription interferes with expression of the copy number control gene, copA, from plasmid R1.

Stougaard P, Light J, Molin S EMBO J. 1982; 1(3):323-8.

PMID: 16453418 PMC: 553043. DOI: 10.1002/j.1460-2075.1982.tb01168.x.

Post-transcriptional control of expression of the repA gene of plasmid R1 mediated by a small RNA molecule.

Light J, Molin S EMBO J. 1983; 2(1):93-8.

PMID: 11894917 PMC: 555093. DOI: 10.1002/j.1460-2075.1983.tb01387.x.

Interaction between the antisense and target RNAs involved in the regulation of IncB plasmid replication.

Siemering K, Praszkier J, Pittard A J Bacteriol. 1993; 175(10):2895-906.

PMID: 7684039 PMC: 204607. DOI: 10.1128/jb.175.10.2895-2906.1993.

Mechanism of binding of the antisense and target RNAs involved in the regulation of IncB plasmid replication.

Siemering K, Praszkier J, Pittard A J Bacteriol. 1994; 176(9):2677-88.

PMID: 7513326 PMC: 205408. DOI: 10.1128/jb.176.9.2677-2688.1994.

Molecular cloning and functional characterization of a copy number control gene (copB) of plasmid R1.

Riise E, Stougaard P, Bindslev B, Nordstrom K, Molin S J Bacteriol. 1982; 151(3):1136-45.

PMID: 7050078 PMC: 220389. DOI: 10.1128/jb.151.3.1136-1145.1982.

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