Interaction Between the Antisense and Target RNAs Involved in the Regulation of IncB Plasmid Replication

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1993 May 1

PMID 7684039

Citations 23

Authors

K R Siemering

J Praszkier

A J Pittard

Affiliations

Soon will be listed here.

Abstract

Physical analysis of RNA I, the small antisense RNA which regulates the replication of IncB miniplasmid pMU720, showed that it is a highly structured molecule containing an imperfectly paired stem closed by a 6-base hairpin loop. Mutational studies revealed that a 3-base sequence in the hairpin loop is critical to the interaction between RNA I and its complementary target in the RepA mRNA (RNA II). Furthermore, a 2-base interior loop in the upper stem was found to play an important role in facilitating effective binding between RNA I and RNA II. From these analyses, a model describing the molecular mechanism of binding between RNA I and RNA II is proposed.

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