Molecular Cloning and Functional Characterization of a Copy Number Control Gene (copB) of Plasmid R1

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Sep 1

PMID 7050078

Citations 19

Authors

E Riise

P Stougaard

B Bindslev

K Nordstrom

S Molin

Affiliations

Soon will be listed here.

Abstract

Deletions or insertions in the copB gene of plasmid R1 result in a copy mutant phenotype. The wild-type copB gene has been cloned on various plasmid vectors. The presence of such chimeric plasmids reduced the copy number of R1 copB mutant plasmids to normal or subnormal levels, indicating the expression of a trans-acting inhibitor activity from the copB chimeras. However, the cloned copB gene did not affect the copy number of wild-type R1, and no incompatibility was exerted by the cloned copB gene against wild-type R1 (or R100). Although the copB gene is not normally required for the incompatibility exerted by copA, it is shown that the CopB function is required for expression of incompatibility by the copA gene from some types of chimeric plasmids. Mutant plasmids that have lost both Cop functions replicate in an uncontrolled fashion.

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Coupling between the basic replicon and the Kis-Kid maintenance system of plasmid R1: modulation by Kis antitoxin levels and involvement in control of plasmid replication.

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Effect of the CopB auxiliary replication control system on stability of maintenance of Par(+) plasmid R1.

Olsson J, Paulsson J, Nordstrom K J Bacteriol. 2003; 186(1):207-11.

PMID: 14679240 PMC: 303431. DOI: 10.1128/JB.186.1.207-211.2004.

Post-transcriptional control of expression of the repA gene of plasmid R1 mediated by a small RNA molecule.

Light J, Molin S EMBO J. 1983; 2(1):93-8.

PMID: 11894917 PMC: 555093. DOI: 10.1002/j.1460-2075.1983.tb01387.x.

Expression of a copy number control gene (copB) of plasmid R1 is constitutive and growth rate dependent.

Light J, Molin S J Bacteriol. 1982; 151(3):1129-35.

PMID: 7050077 PMC: 220388. DOI: 10.1128/jb.151.3.1129-1135.1982.

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