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F G Hansen

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Articles 55
Citations 2125
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Recent Articles
1.
Nielsen H, Hansen F
J Microsc . 2010 Aug; 239(3):194-9. PMID: 20701657
Direct measurements of cells from photo micrographs are becoming increasingly used when investigating the position and/or distribution of chromosomal loci in bacteria. In general, these measurements have been done manually,...
2.
Atlung T, Hansen F
J Bacteriol . 2002 Mar; 184(7):1843-50. PMID: 11889089
Flow cytometric analysis showed that the hns205 and hns206 mutants, lacking the abundant nucleoid-associated protein H-NS, have decreased origin concentration, as well as a low number of origins per cell...
3.
Atlung T, Nielsen H, Hansen F
Mol Genet Genomics . 2002 Jan; 266(5):873-81. PMID: 11810263
The nucleotide sequence of rpoS, the gene for the stress sigma factor, was determined in 13 different K12 strains of Escherichia coli. The results indicate that the original K12 isolate...
4.
Ostergaard H, Henriksen A, Hansen F, Winther J
EMBO J . 2001 Nov; 20(21):5853-62. PMID: 11689426
To visualize the formation of disulfide bonds in living cells, a pair of redox-active cysteines was introduced into the yellow fluorescent variant of green fluorescent protein. Formation of a disulfide...
5.
von Freiesleben U, Krekling M, Hansen F, Lobner-Olesen A
EMBO J . 2000 Nov; 19(22):6240-8. PMID: 11080169
The minimal time between successive initiations on the same origin (the eclipse) in Escherichia coli was determined to be approximately 25-30 min. An inverse relationship was found between the length...
6.
von Freiesleben U, Rasmussen K, Atlung T, Hansen F
Mol Microbiol . 2000 Sep; 37(5):1087-93. PMID: 10972827
IHF (integration host factor) mutants exhibit asynchronous initiation of chromosome replication from oriC as determined from flow cytometric analysis of cultures where RNA synthesis was inhibited with rifampicin. However, the...
7.
Nyborg M, Atlung T, Skovgaard O, Hansen F
Mol Microbiol . 2000 Mar; 35(5):1202-10. PMID: 10712700
Multicopy dnaA(Ts) strains carrying the dnaA5 or dnaA46 allele are high-temperature resistant but are cold sensitive for colony formation. The DnaA5 and DnaA46 proteins both have an A184-->V change in...
8.
Atlung T, Hansen F
J Bacteriol . 1999 Sep; 181(18):5557-62. PMID: 10482494
Expression of the dnaA gene continues in the lag phase following a temperature downshift, indicating that DnaA is a cold shock protein. Steady-state DnaA protein concentration increases at low temperatures,...
9.
Christensen B, Atlung T, Hansen F
J Bacteriol . 1999 Apr; 181(9):2683-8. PMID: 10217754
The binding of DnaA protein to its DNA binding sites-DnaA boxes-in the chromosomal oriC region is essential for initiation of chromosome replication. In this report, we show that additional DnaA...
10.
Atlung T, Christensen B, Hansen F
Plasmid . 1999 Mar; 41(2):110-9. PMID: 10087214
The copy number per cell mass of plasmid pBR322 and a rom- derivative was measured as a function of generation time. In fast growing cells the copy number per cell...