Cloning of DNA of the RpoBC Operon from the Chromosome of Escherichia Coli K12

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Feb 1

PMID 6246404

Citations 18

Authors

A Newman

R S Hayward

Affiliations

Soon will be listed here.

Abstract

We provide evidence that, in terms of transcriptional organisation, the rpoBC operon carried by lambdarifd 18 accurately represents the corresponding region of the E. coli K12 chromosome. A restriction fragment of E. coli K12 chromosomal DNA carrying the genes rpoBC (encoding the beta and beta' subunits of RNA polymerase) and rplL (coding for ribosomal proteins L7/L12) was cloned in a lambda vector, and the resulting phage tested for gene expression. In common with the corresponding fragment of lambdarifd 18 DNA, the chromosomal fragment has no strong promoter for rplL or rpoBC transcription. Another new phage was constructed by adding, to the restriction fragment carrying the rplL rpoBC structural genes from lambdarifd 18, a sequence from the E. coli K12 chromosome which includes a promoter for these genes. As in lambdarifd 18 itself, this promoter is shared with rplJ but not with rplKA. The properties of the latter phage also show that the dominant rifampicin-resistance characteristic of lambdarifd 18 results from more than one mutation.

Citing Articles

Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Berlyn M Microbiol Mol Biol Rev. 1998; 62(3):814-984.

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In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coli.

Bruckner R, Matzura H Mol Gen Genet. 1981; 183(2):277-82.

PMID: 7035827 DOI: 10.1007/BF00270629.

Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VI. A redundant region in the beta polypeptide.

Nene V, Glass R Mol Gen Genet. 1984; 196(1):64-7.

PMID: 6384732 DOI: 10.1007/BF00334093.

Linkage map of Escherichia coli K-12, edition 7.

Bachmann B Microbiol Rev. 1983; 47(2):180-230.

PMID: 6348505 PMC: 281571. DOI: 10.1128/mr.47.2.180-230.1983.

RNA polymerase rifampicin resistance mutations in Escherichia coli: sequence changes and dominance.

Ovchinnikov Y, Monastyrskaya G, Guriev S, Kalinina N, Sverdlov E, Gragerov A Mol Gen Genet. 1983; 190(2):344-8.

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