Transformation of Escherichia Coli by a Specific DNA Restriction Fragment

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1977 Oct 20

PMID 337130

Citations 4

Authors

S M Schweitzer

H Matzura

Affiliations

Soon will be listed here.

Abstract

Specific transformation of a rifampicin sensitive strain of Escherichia coli to rifampicin resistance has been performed by a single, defined DNA restriction fragment carrying the genetic information for the beta subunit of E. coli RNA polymerase. In this transformation the transforming genetic character has been substituted for the corresponding recipient gene locus by recombination. The value of the described transformation system for locating genetic markers on DNA restriction fragments is discussed in comparison to previously reported in vitro systems.

Citing Articles

Cloning of DNA of the rpoBC operon from the chromosome of Escherichia coli K12.

Newman A, Hayward R Mol Gen Genet. 1980; 177(3):527-33.

PMID: 6246404 DOI: 10.1007/BF00271493.

Evidence for co-transcription of the RNA polymerase genes rpoBC with a ribosomal protein gene of escherichia coli.

Newman A, Linn T, Hayward R Mol Gen Genet. 1979; 169(2):195-204.

PMID: 375008 DOI: 10.1007/BF00271671.

Contranscription of genes for RNA polymerase subunits beta and beta' with genes for ribosomal proteins in Escherichia coli.

Yamamoto M, Nomura M Proc Natl Acad Sci U S A. 1978; 75(8):3891-5.

PMID: 358203 PMC: 392894. DOI: 10.1073/pnas.75.8.3891.

Control features within the rplJL-rpoBC transcription unit of Escherichia coli.

Barry G, Squires C, Squires C Proc Natl Acad Sci U S A. 1979; 76(10):4922-6.

PMID: 116224 PMC: 413050. DOI: 10.1073/pnas.76.10.4922.

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