Genetic Studies on the Beta Subunit of Escherichia Coli RNA Polymerase. VIII. Localisation of a Region Involved in Promoter Selectivity

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1986 Jun 1

PMID 3528751

Citations 13

Authors

R E Glass

S T Jones

V Nene

T Nomura

N Fujita

A Ishihama

Affiliations

Soon will be listed here.

Abstract

We have previously isolated an E. coli derivative carrying a small internal deletion (delta(rpoB)1570-1) of the beta structural gene. This RNA polymerase deletion mutant has no noticeable phenotype other than a slightly increased generation time in minimal medium. The deletion, which removes about 165 bp, has been localised to between codons 965 and 1,083, indicating it excises part of a tandem repeat structure present in the C-terminal region of beta. Analysis in vitro of purified RNA polymerase from the deletion mutant indicates that this enzyme has an altered promoter selectivity. These observations allow localisation of a site on the beta polypeptide of E. coli RNA polymerase involved in transcriptional initiation.

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