Biogenesis of Mitochondria: the Mitochondrial Gene (aap1) Coding for Mitochondrial ATPase Subunit 8 in Saccharomyces Cerevisiae

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1983 Jul 11

PMID 6223276

Citations 51

Authors

I G Macreadie

C E Novitski

R J Maxwell

U John

B G Ooi

G L McMullen

H B Lukins

A W Linnane

P Nagley

Affiliations

Soon will be listed here.

Abstract

A mitochondrial gene (denoted aap1) in Saccharomyces cerevisiae has been characterized by nucleotide sequence analysis of a region of mtDNA between the oxi3 and oli2 genes. The reading frame of the aap1 gene specifies a hydrophobic polypeptide containing 48 amino acids. The functional nature of this reading frame was established by sequence analysis of a series of mit- mutants and revertants. Evidence is presented that the aap1 gene codes for a mitochondrially synthesized polypeptide associated with the mitochondrial ATPase complex. This polypeptide (denoted subunit 8) is a proteolipid whose size has been previously assumed to be 10 kilodaltons based on its mobility on SDS-polyacrylamide gels, but the sequence of the aap1 gene predicts a molecular weight of 5,815 for this protein.

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