Definition of T Cell Idiotypes Using Anti-idiotypic Antisera Produced by Immunization with T Cell Clones

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1982 Apr 1

PMID 6174671

Citations 18

Authors

A J Infante

P D Infante

S Gillis

C G Fathman

Affiliations

Soon will be listed here.

Abstract

Alloreactive T cell clones with distinct specificities were used to raise anti-idiotypic antisera via an F1 anti-(parent anti-F1) protocol. Antisera were raised that could stimulate the proliferation of the appropriate T cell clone, but not other clones. The active fraction of the antisera for T cell proliferation was immunoglobulin. In addition to proliferation, an anti-idiotypic antiserum could induce the appropriate T cell clone to secrete substantial amounts of interleukin 2 (IL-2). Production of IL-2 appeared independent of the involvement of accessory cells. These accessory cells may be unnecessary for IL-2 production in our assay, or their effect may be produced by anti-idiotype. Thus, anti-idiotype may provide two or more specific T cell signals.

Citing Articles

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Human auto-antiidiotypes regulating T cell-mediated reactivity to tetanus toxoid.

Saxon A, Barnett E J Clin Invest. 1984; 73(2):342-8.

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