Monoclonal Antibodies That Recognize the Product Controlled by a Gene in the I-J Subregion of the Mouse H-2 Complex

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1981 Nov 1

PMID 6170715

Citations 17

Authors

M Kanno

S Kobayashi

T Tokuhisa

I Takei

N Shinohara

M Taniguchi

Affiliations

Soon will be listed here.

Abstract

The B cell hybridomas producing monoclonal antibodies (E10, D7, F4, H6, and D4) were established by the fusion of P3U1 or NS-1 murine myeloma cell lines and spleen cells of B10.A(5R) mice hyperimmunized with mitomycin C-treated B10.A(3R) spleen and thymus cells. Two types of monoclonal antibodies specific for the products controlled by a gene in the I-Jb subregion of the H-2 complex were characterized: one specific for the private type of I-Jb determinant, the other recognizing the cross-reactive determinant between the I-Jb and I-Jd products. By using these monoclonal reagents, the I-J-encoded product on the antigen-specific suppressor T cells was found to be expressed on their soluble suppressor factors. Furthermore, the I-Jb products were successfully detected not only on the T cell hybridoma with suppressor activity specific for keyhole limpet hemocyanin (KLH), but also on KLH-primed suppressor T cells enriched by antigen-coated petri dishes and concanavalin A-induced thymocyte blasts of C57BL/6 mice by complement-dependent cytotoxic assays and membrane fluorescence techniques.

Citing Articles

Association of the "major histocompatibility complex subregion" I-J determinant with bioactive glycosylation-inhibiting factor.

Nakano T, Liu Y, Mikayama T, Watarai H, Taniguchi M, Ishizaka K Proc Natl Acad Sci U S A. 1995; 92(20):9196-200.

PMID: 7568100 PMC: 40951. DOI: 10.1073/pnas.92.20.9196.

Murine T cell-specific Ia antigens: monoclonal antibodies define an I-A-encoded T lymphocyte structure.

Hayes C, Hullett D Proc Natl Acad Sci U S A. 1982; 79(11):3594-8.

PMID: 6980412 PMC: 346469. DOI: 10.1073/pnas.79.11.3594.

Regulation of immune responses by I-J gene products. I. Production and characterization of anti-I-J monoclonal antibodies.

Waltenbaugh C J Exp Med. 1981; 154(5):1570-83.

PMID: 6795302 PMC: 2186531. DOI: 10.1084/jem.154.5.1570.

Regulation of the immune response to antigens on the malignant cell surface.

Drebin J, Perry L, Carter R, Greene M Springer Semin Immunopathol. 1982; 5(2):175-92.

PMID: 6293112 DOI: 10.1007/BF00199795.

I-J subregion and suppressor signals.

Bromberg J, Tominaga A, Takaoki M, Greene M Surv Immunol Res. 1982; 1(1):67-75.

PMID: 6227066 DOI: 10.1007/BF02918242.

References

Greene M, Pierres A, Dorf M, Benacerraf B . The I-J subregion codes for determinats on suppressor factor(s) which limit the contact sensitivity response to picryl chloride. J Exp Med. 1977; 146(1):293-6. PMC: 2180745. DOI: 10.1084/jem.146.1.293. View

Taniguchi M, Saito T, Takei I, Tokuhisa T . Presence of interchain disulfide bonds between two gene products that compose the secreted form of an antigen-specific suppressor factor. J Exp Med. 1981; 153(6):1672-7. PMC: 2186189. DOI: 10.1084/jem.153.6.1672. View

Taniguchi M, Miller J . Enrichment of specific suppressor T cells and characterization of their surface markers. J Exp Med. 1977; 146(5):1450-4. PMC: 2180969. DOI: 10.1084/jem.146.5.1450. View

Tada T, Takemori T, Okumura K, Nonaka M, Tokuhisa T . Two distinct types of helper T cells involved in the secondary antibody response: independent and synergistic effects of Ia- and Ia+ helper T cells. J Exp Med. 1978; 147(2):446-58. PMC: 2184496. DOI: 10.1084/jem.147.2.446. View

Axen R, Porath J, Ernback S . Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967; 214(5095):1302-4. DOI: 10.1038/2141302a0. View

Klein J . List of congenic lines of mice. I. Lines with differences at alloantigen loci. Transplantation. 1973; 15(1):137-53. DOI: 10.1097/00007890-197301000-00021. View

Shreffler D, David C . The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975; 20:125-95. DOI: 10.1016/s0065-2776(08)60208-4. View

Murphy D, Okumura K, Herzenberg L, McDevitt H . A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes. J Exp Med. 1976; 144(3):699-712. PMC: 2190409. DOI: 10.1084/jem.144.3.699. View

Tada T, Taniguchi M, David C . Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex. J Exp Med. 1976; 144(3):713-25. PMC: 2190403. DOI: 10.1084/jem.144.3.713. View

10.

Frelinger J, NIEDERHUBER J, Shreffler D . Effects of anti-Ia sera on mitogenic responses. III. Mapping the genes controlling the expression of Ia determinants on concanavalin A-reactive cells to the I-J subregion of the H-2 gene complex. J Exp Med. 1976; 144(4):1141-6. PMC: 2190424. DOI: 10.1084/jem.144.4.1141. View

11.

Theze J, Kapp J, Benacerraf B . Immunosuppressive factor(s) extracted from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) III. Immunochemical properties of the GAT-specific suppressive factor. J Exp Med. 1977; 145(4):839-56. PMC: 2180626. DOI: 10.1084/jem.145.4.839. View

12.

Theze J, Waltenbaugh C, Dorf M, Benacerraf B . Immunosuppressive factor(s) specific for L-glutamic acid50-L-tyrosine50 (GT) II. Presence of I-J determinants on the GT-suppressive factor. J Exp Med. 1977; 146(1):287-92. PMC: 2180744. DOI: 10.1084/jem.146.1.287. View

13.

Oi V, Jones P, Goding J, Herzenberg L . Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978; 81:115-20. DOI: 10.1007/978-3-642-67448-8_18. View

14.

Hayes C, Bach F . T-cell-specific murine Ia antigens: serology of I-J and I-E subregion specificities. J Exp Med. 1978; 148(3):692-703. PMC: 2184995. DOI: 10.1084/jem.148.3.692. View

15.

Germain R, Ju S, Kipps T, Benacerraf B, Dorf M . Shared idiotypic determinants on antibodies and T-cell-derived suppressor factor specific for the random terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10. J Exp Med. 1979; 149(3):613-22. PMC: 2184820. DOI: 10.1084/jem.149.3.613. View

16.

Taniguchi M, Saito T, Tada T . Antigen-specific suppressive factor produced by a transplantable I-J bearing T-cell hybridoma. Nature. 1979; 278(5704):555-8. DOI: 10.1038/278555a0. View

17.

NIEDERHUBER J, Allen P, Mayo L . The expression of Ia antigenic determinants on macrophages required for the in vitro antibody response. J Immunol. 1979; 122(4):1342-9. View

18.

Taniguchi M, Takei I, Tada T . Functional and molecular organisation of an antigen-specific suppressor factor from a T-cell hybridoma. Nature. 1980; 283(5743):227-8. DOI: 10.1038/283227a0. View

19.

Tada T, Okumura K . The role of antigen-specific T cell factors in the immune response. Adv Immunol. 1979; 28:1-87. DOI: 10.1016/s0065-2776(08)60799-3. View

20.

Okumura K, Takemori T, Tokuhisa T, Tada T . Specific enrichment of the suppressor T cell bearing I-J determinants: parallel functional and serological characterizations. J Exp Med. 1977; 146(5):1234-45. PMC: 2180967. DOI: 10.1084/jem.146.5.1234. View