Presence of Interchain Disulfide Bonds Between Two Gene Products That Compose the Secreted Form of an Antigen-specific Suppressor Factor

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1981 Jun 1

PMID 6166720

Citations 27

Authors

M Taniguchi

T Saito

I Takei

T Tokuhisa

Affiliations

Soon will be listed here.

Abstract

The secreted form of the suppressor T cell factor specific for keyhole limpet hemocyanin derived from the hybridoma 34S-704 was found to consist of the two distinct polypeptide chains, i.e., the antigen-binding and the I-J-encoded chains. They were linked in covalent association with disulfide bonds. The two chains were cleaved by the reduction with dithiothreitol and were easy to reconstitute the active form of TsF. The association of the two distinct chains was suggested to be essential for the expression of the TsF activity.

Citing Articles

Special regulatory T-cell review: A rose by any other name: from suppressor T cells to Tregs, approbation to unbridled enthusiasm.

Germain R Immunology. 2007; 123(1):20-7.

PMID: 18154615 PMC: 2433291. DOI: 10.1111/j.1365-2567.2007.02779.x.

Antigen-specific suppressor factor: missing pieces in the puzzle.

OHara Jr R Immunol Res. 1995; 14(4):252-62.

PMID: 8722042 DOI: 10.1007/BF02935623.

Interactions between molecules (subfactors) released by different T cell sets that yield a complete factor with biological (suppressive) activity.

Ptak W, Rosenstein R, GERSHON R Proc Natl Acad Sci U S A. 1982; 79(7):2375-8.

PMID: 6980420 PMC: 346196. DOI: 10.1073/pnas.79.7.2375.

Antigen-specific suppressor T cell interactions. II. Characterization of two different types of suppressor T cell factors specific for L-glutamic acid50-L-tyrosine50 (GT) and L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT).

Kapp J, Sorensen C, Pierce C J Exp Med. 1983; 158(6):1962-78.

PMID: 6606014 PMC: 2187171. DOI: 10.1084/jem.158.6.1962.

Diffusible suppressor factor from splenic macrophages in murine plasmacytoma.

Chen Y, Heller P Clin Exp Immunol. 1984; 57(1):171-7.

PMID: 6378461 PMC: 1536073.

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