Monoclonal Antibody Against an Ir Gene Product?

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1980 Oct 1

PMID 6158546

Citations 91

Authors

E A Lerner

L A Matis

C A Janeway Jr

P P Jones

R H Schwartz

D B Murphy

Affiliations

Soon will be listed here.

Abstract

Genetic, biochemical, and functional studies have been performed using a monoclonal antibody, Y-17, directed at a conformational or combinatorial determinant formed by certain Ae:E alpha complexes. This determinant appears to be a marker present on a subset of B cells as well as on non-T and non-B spleen cells. Besides Ae and E alpha chains, Y-17 precipitates a third chain that is indistinguishable from the A alpha chain in two-dimensional gels. This results suggests additional combinatorial complexity in the generation of I-region encoded antigens. Y-17 can inhibit the response of T cells to Ae:E alpha determinants in mixed lymphocyte cultures. Furthermore, Y-17 blocks antigen-specific T cell proliferative responses to GLPhe and pigeon cytochrome c which have been shown to require the Ae:E alpha complex as a restriction element for antigen presentation. These results provide strong evidence for the molecular identity of Ia antigens, Ir-gene products and Lad antigens.

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