» Articles » PMID: 22984083

Cutting Edge: NADPH Oxidase Modulates MHC Class II Antigen Presentation by B Cells

Overview
Journal J Immunol
Date 2012 Sep 18
PMID 22984083
Citations 18
Authors
Affiliations
Soon will be listed here.
Abstract

Phagocyte NADPH oxidase plays a key role in pathogen clearance via reactive oxygen species (ROS) production. Defects in oxidase function result in chronic granulomatous disease with hallmark recurrent microbial infections and inflammation. The oxidase's role in the adaptive immune response is not well understood. Class II presentation of cytoplasmic and exogenous Ag to CD4(+) T cells was impaired in human B cells with reduced oxidase p40(phox) subunit expression. Naturally arising mutations, which compromise p40(phox) function in a chronic granulomatous disease patient, also perturbed class II Ag presentation and intracellular ROS production. Reconstitution of patient B cells with a wild-type, but not a mutant, p40(phox) allele restored exogenous Ag presentation and intracellular ROS generation. Remarkably, class II presentation of epitopes from membrane Ag was robust in p40(phox)-deficient B cells. These studies reveal a role for NADPH oxidase and p40(phox) in skewing epitope selection and T cell recognition of self Ag.

Citing Articles

A New Patient with p40 Deficiency and Chronic Immune Thrombocytopenia.

Neehus A, Fusaro M, Levy R, Bustamante J J Clin Immunol. 2023; 43(6):1173-1177.

PMID: 37198372 PMC: 10354143. DOI: 10.1007/s10875-023-01498-4.


Exercise Cuts Both Ways with ROS in Remodifying Innate and Adaptive Responses: Rewiring the Redox Mechanism of the Immune System during Exercise.

Thirupathi A, Gu Y, Pinho R Antioxidants (Basel). 2021; 10(11).

PMID: 34829717 PMC: 8615250. DOI: 10.3390/antiox10111846.


Beyond the Extra Respiration of Phagocytosis: NADPH Oxidase 2 in Adaptive Immunity and Inflammation.

Mortimer P, Mc Intyre S, Thomas D Front Immunol. 2021; 12:733918.

PMID: 34539670 PMC: 8440999. DOI: 10.3389/fimmu.2021.733918.


Attenuated Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice.

Troxell B, Mendoza M, Ali R, Koci M, Hassan H Vaccines (Basel). 2020; 8(4).

PMID: 33153043 PMC: 7711481. DOI: 10.3390/vaccines8040646.


NOX2ko Mice Show Largely Increased Expression of a Mutated NOX2 mRNA Encoding an Inactive NOX2 Protein.

Gollner M, Ihrig-Biedert I, Petermann V, Saurin S, Oelze M, Kroller-Schon S Antioxidants (Basel). 2020; 9(11).

PMID: 33114493 PMC: 7692237. DOI: 10.3390/antiox9111043.


References
1.
Richards S, Clark E . BCR-induced superoxide negatively regulates B-cell proliferation and T-cell-independent type 2 Ab responses. Eur J Immunol. 2009; 39(12):3395-403. PMC: 3419136. DOI: 10.1002/eji.200939587. View

2.
Lich J, Jayne J, Zhou D, Elliott J, Blum J . Editing of an immunodominant epitope of glutamate decarboxylase by HLA-DM. J Immunol. 2003; 171(2):853-9. DOI: 10.4049/jimmunol.171.2.853. View

3.
Groemping Y, Rittinger K . Activation and assembly of the NADPH oxidase: a structural perspective. Biochem J. 2004; 386(Pt 3):401-16. PMC: 1134858. DOI: 10.1042/BJ20041835. View

4.
Olsson L, Lindqvist A, Kallberg H, Padyukov L, Burkhardt H, Alfredsson L . A case-control study of rheumatoid arthritis identifies an associated single nucleotide polymorphism in the NCF4 gene, supporting a role for the NADPH-oxidase complex in autoimmunity. Arthritis Res Ther. 2007; 9(5):R98. PMC: 2212587. DOI: 10.1186/ar2299. View

5.
Barbey C, Tiercy J, Fairweather N, Niemann H, Seger R, Corradin G . Processing and presentation of tetanus toxin by antigen-presenting cells from patients with chronic granulomatous disease (CGD) to human specific T cell clones are not impaired. Clin Exp Immunol. 1994; 95(2):227-31. PMC: 1534922. DOI: 10.1111/j.1365-2249.1994.tb06515.x. View