Evidence for a Functional Interaction Between the Beta Chain of Major Histocompatibility Complex Class II and the T Cell Receptor Alpha Chain During Recognition of a Bacterial Superantigen

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1994 Nov 1

PMID 7964468

Citations 7

Authors

A M Deckhut

Y Chien

M A Blackman

D L Woodland

Affiliations

Soon will be listed here.

Abstract

Several studies have suggested that there is a direct interaction between the T cell receptor (TCR) and the major histocompatibility complex (MHC) molecule during T cell recognition of superantigen. To further investigate this possibility, we have analyzed T cell recognition of a bacterial superantigen, Staphylococcal enterotoxin B (SEB), presented by a series of mutant murine I-Ek molecules in which residues of either the alpha or beta chain predicted to interact with the TCR have been substituted. Individual T cell hybridomas gave distinct patterns of responsiveness to SEB presented by the I-E beta k mutants that could not be attributed to differences in the binding of SEB to the mutants. This effect appeared to be dependent on the TCR-alpha chain because some of these hybridomas expressed identical TCR transgenic beta chains. In contrast, none of the hybridomas gave distinct patterns of responsiveness to SEB presented by the I-E alpha k mutants. Taken together, these observations support the idea that there is a functional interaction between the alpha chain of the TCR and the beta chain of the MHC class II molecule. The data also support the idea that this interaction might enhance superantigen recognition in some cases.

Citing Articles

Role of the T cell receptor alpha-chain in superantigen recognition.

Blackman M, Woodland D Immunol Res. 1996; 15(2):98-113.

PMID: 8839779 DOI: 10.1007/BF02918500.

Different superantigens interact with distinct sites in the Vbeta domain of a single T cell receptor.

Hong S, Waterbury G, Janeway Jr C J Exp Med. 1996; 183(4):1437-46.

PMID: 8666902 PMC: 2192526. DOI: 10.1084/jem.183.4.1437.

Major histocompatibility complex class II-associated peptides control the presentation of bacterial superantigens to T cells.

Wen R, Cole G, Surman S, Blackman M, Woodland D J Exp Med. 1996; 183(3):1083-92.

PMID: 8642250 PMC: 2192316. DOI: 10.1084/jem.183.3.1083.

Isolation of HLA-DR1.(staphylococcal enterotoxin A)2 trimers in solution.

Tiedemann R, Urban R, Strominger J, Fraser J Proc Natl Acad Sci U S A. 1995; 92(26):12156-9.

PMID: 8618863 PMC: 40315. DOI: 10.1073/pnas.92.26.12156.

Genetic background and environment contribute synergistically to the onset of autoimmune diseases.

Luppi P, Rossiello M, Faas S, Trucco M J Mol Med (Berl). 1995; 73(8):381-93.

PMID: 8528740 DOI: 10.1007/BF00240137.

References

Lerner E, Matis L, Janeway Jr C, Jones P, Schwartz R, Murphy D . Monoclonal antibody against an Ir gene product?. J Exp Med. 1980; 152(4):1085-101. PMC: 2185961. DOI: 10.1084/jem.152.4.1085. View

Surman S, Deckhut A, Blackman M, Woodland D . MHC-specific recognition of a bacterial superantigen by weakly reactive T cells. J Immunol. 1994; 152(10):4893-902. View

Gascoigne N, Ames K . Direct binding of secreted T-cell receptor beta chain to superantigen associated with class II major histocompatibility complex protein. Proc Natl Acad Sci U S A. 1991; 88(2):613-6. PMC: 50862. DOI: 10.1073/pnas.88.2.613. View

Herman A, Labrecque N, Thibodeau J, Marrack P, Kappler J, Sekaly R . Identification of the staphylococcal enterotoxin A superantigen binding site in the beta 1 domain of the human histocompatibility antigen HLA-DR. Proc Natl Acad Sci U S A. 1991; 88(22):9954-8. PMC: 52845. DOI: 10.1073/pnas.88.22.9954. View

Janeway Jr C . Selective elements for the V beta region of the T cell receptor: Mls and the bacterial toxic mitogens. Adv Immunol. 1991; 50:1-53. DOI: 10.1016/s0065-2776(08)60821-4. View

Kappler J, Herman A, Clements J, Marrack P . Mutations defining functional regions of the superantigen staphylococcal enterotoxin B. J Exp Med. 1992; 175(2):387-96. PMC: 2119125. DOI: 10.1084/jem.175.2.387. View

Karp D, Long E . Identification of HLA-DR1 beta chain residues critical for binding staphylococcal enterotoxins A and E. J Exp Med. 1992; 175(2):415-24. PMC: 2119122. DOI: 10.1084/jem.175.2.415. View

Vacchio M, Kanagawa O, Tomonari K, Hodes R . Influence of T cell receptor V alpha expression on Mlsa superantigen-specific T cell responses. J Exp Med. 1992; 175(5):1405-8. PMC: 2119212. DOI: 10.1084/jem.175.5.1405. View

SMITH H, Le P, Woodland D, Blackman M . T cell receptor alpha-chain influences reactivity to Mls-1 in V beta 8.1 transgenic mice. J Immunol. 1992; 149(3):887-96. View

10.

Swaminathan S, Furey W, Pletcher J, Sax M . Crystal structure of staphylococcal enterotoxin B, a superantigen. Nature. 1992; 359(6398):801-6. DOI: 10.1038/359801a0. View

11.

Woodland D, SMITH H, Surman S, Le P, Wen R, Blackman M . Major histocompatibility complex-specific recognition of Mls-1 is mediated by multiple elements of the T cell receptor. J Exp Med. 1993; 177(2):433-42. PMC: 2190884. DOI: 10.1084/jem.177.2.433. View

12.

Waanders G, Lussow A, MacDonald H . Skewed T cell receptor V alpha repertoire among superantigen reactive murine T cells. Int Immunol. 1993; 5(1):55-61. DOI: 10.1093/intimm/5.1.55. View

13.

Woodland D, Blackman M . How do T-cell receptors, MHC molecules and superantigens get together?. Immunol Today. 1993; 14(5):208-12. DOI: 10.1016/0167-5699(93)90164-G. View

14.

Ehrich E, Devaux B, Rock E, Jorgensen J, Davis M, Chien Y . T cell receptor interaction with peptide/major histocompatibility complex (MHC) and superantigen/MHC ligands is dominated by antigen. J Exp Med. 1993; 178(2):713-22. PMC: 2191102. DOI: 10.1084/jem.178.2.713. View

15.

Labrecque N, Thibodeau J, Sekaly R . T-cell receptor recognition of superantigens: another view. Res Immunol. 1993; 144(3):175-80. DOI: 10.1016/0923-2494(93)80113-d. View

16.

Fraser J, Hudson K . Superantigens--remnants of a past process?. Res Immunol. 1993; 144(3):188-93; discussion 214-22. DOI: 10.1016/0923-2494(93)80116-g. View

17.

Jardetzky T, Brown J, Gorga J, Stern L, Urban R, Chi Y . Three-dimensional structure of a human class II histocompatibility molecule complexed with superantigen. Nature. 1994; 368(6473):711-8. DOI: 10.1038/368711a0. View

18.

Woodland D, Happ M, Bill J, Palmer E . Requirement for cotolerogenic gene products in the clonal deletion of I-E reactive T cells. Science. 1990; 247(4945):964-7. DOI: 10.1126/science.1968289. View