Rare Internal C4A4 Repeats in the Micronuclear Genome of Oxytricha Fallax

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1984 Dec 1

PMID 6098818

Citations 6

Authors

D Dawson

G Herrick

Affiliations

Soon will be listed here.

Abstract

Approximately 20,000 different short, linear, macronuclear DNA molecules are derived from micronuclear sequences of Oxytricha fallax after conjugation. These macronuclear DNAs are terminated at both ends by 20 base pairs of the sequence 5'-dC4A4-3'. Sequences homologous to this repeat (C4A4+) are also abundant in the micronuclear chromosomes, but most reside at their telomeres. Here we show that nontelomeric C4A4 clusters of 20 base pairs or longer exist in only a few hundred copies per micronuclear genome. This demonstrates that nearly none of the 20,000 sequence blocks of micronuclear DNA destined to be macronuclear DNA molecules can be flanked by full-length (20-base pair) C4A4 clusters, and therefore C4A4 repeats must be added to most, if not all, macronuclear telomeres during macronuclear development. Six internal micronuclear C4A4+ loci were cloned, and their structural relationships with macronuclear and micronuclear sequences were examined. The possible origins and functions of these rare, micronuclear internal C4A4 loci are discussed.

Citing Articles

Telomere formation on macronuclear chromosomes of Oxytricha trifallax and O. fallax: alternatively processed regions have multiple telomere addition sites.

Williams K, Doak T, Herrick G BMC Genet. 2002; 3:16.

PMID: 12199911 PMC: 128808. DOI: 10.1186/1471-2156-3-16.

Interactions between telomerase and primase physically link the telomere and chromosome replication machinery.

Ray S, Karamysheva Z, Wang L, Shippen D, Price C Mol Cell Biol. 2002; 22(16):5859-68.

PMID: 12138196 PMC: 133977. DOI: 10.1128/MCB.22.16.5859-5868.2002.

The DNA of ciliated protozoa.

Prescott D Microbiol Rev. 1994; 58(2):233-67.

PMID: 8078435 PMC: 372963. DOI: 10.1128/mr.58.2.233-267.1994.

Autonomous replication and addition of telomerelike sequences to DNA microinjected into Paramecium tetraurelia macronuclei.

Gilley D, PREER Jr J, Aufderheide K, Polisky B Mol Cell Biol. 1988; 8(11):4765-72.

PMID: 3211128 PMC: 365568. DOI: 10.1128/mcb.8.11.4765-4772.1988.

Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova.

Klobutcher L, Cahill K, Ribas-Aparicio R Mol Cell Biol. 1986; 6(11):3606-13.

PMID: 3025603 PMC: 367120. DOI: 10.1128/mcb.6.11.3606-3613.1986.

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