Gene-sized Macronuclear DNA Molecules Are Clustered in Micronuclear Chromosomes of the Ciliate Oxytricha Nova

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1986 Nov 1

PMID 3025603

Citations 8

Authors

L A Klobutcher

K Cahill

R M Ribas-Aparicio

Affiliations

Soon will be listed here.

Abstract

Following the sexual phase of its life cycle, the hypotrichous ciliate Oxytricha nova transforms a copy of its chromosomal micronucleus into a macronucleus containing short, linear DNA molecules with an average size of 2.2 kilobase pairs. In addition, more than 90% of the DNA sequences in the micronuclear genome are eliminated during this process. We have examined the organization of macronuclear DNA molecules in the micronuclear chromosomes. Macronuclear DNA molecules were found to be clustered and separated by less than 550 base pairs in two cloned segments of micronuclear DNA. Recombinant clones of two macronuclear DNA molecules that are adjacent in the micronucleus were also isolated and examined by DNA sequencing. The two macronuclear DNA molecules were found to be separated by only 90 base pairs in the micronuclear genome.

Citing Articles

Organization of the micronuclear genome of oxytricha nova.

Jahn C, Prescott K, Waggener M Genetics. 1988; 120(1):123-34.

PMID: 17246474 PMC: 1203484. DOI: 10.1093/genetics/120.1.123.

Conserved linkage of two genes on the same macronuclear chromosome in spirotrichous ciliates.

McFarland C, Chang W, Kuo S, Landweber L Chromosoma. 2006; 115(2):129-38.

PMID: 16520956 DOI: 10.1007/s00412-005-0040-6.

Differential replication and DNA elimination in the polytene chromosomes of Euplotes crassus.

Frels J, Tebeau C, Doktor S, Jahn C Mol Biol Cell. 1996; 7(5):755-68.

PMID: 8744949 PMC: 275928. DOI: 10.1091/mbc.7.5.755.

The DNA of ciliated protozoa.

Prescott D Microbiol Rev. 1994; 58(2):233-67.

PMID: 8078435 PMC: 372963. DOI: 10.1128/mr.58.2.233-267.1994.

Alternative use of chromosome fragmentation sites in the ciliated protozoan Oxytricha nova.

Klobutcher L, Huff M, Gonye G Nucleic Acids Res. 1988; 16(1):251-64.

PMID: 2829118 PMC: 334624. DOI: 10.1093/nar/16.1.251.

References

Ammermann D . Morphology and development of the macronuclei of the ciliates Stylonychia mytilus and Euplotes aediculatus. Chromosoma. 1971; 33(2):209-38. DOI: 10.1007/BF00285634. View

Prescott D, Murti K, Bostock C . Genetic apparatus of Stylonychia sp. Nature. 1973; 242(5400):576, 597-600. DOI: 10.1038/242576a0. View

Judd B, YOUNG M . An examination of the one cistron: one chromomere concept. Cold Spring Harb Symp Quant Biol. 1974; 38:573-9. DOI: 10.1101/sqb.1974.038.01.061. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Spear B, Lauth M . Polytene chromosomes of Oxytricha: biochemical and morphological changes during macronuclear development in a ciliated protozoan. Chromosoma. 1976; 54(1):1-13. DOI: 10.1007/BF00331828. View

Lauth M, Spear B, HEUMANN J, Prescott D . DNA of ciliated protozoa: DNA sequence diminution during macronuclear development of Oxytricha. Cell. 1976; 7(1):67-74. DOI: 10.1016/0092-8674(76)90256-7. View

Benton W, Davis R . Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977; 196(4286):180-2. DOI: 10.1126/science.322279. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

10.

Bolivar F . Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978; 4(2):121-36. DOI: 10.1016/0378-1119(78)90025-2. View

11.

Kuhn S, Fritz H, Starlinger P . Close vicinity of IS1 integration sites in the leader sequence of the gal operon of E. coli. Mol Gen Genet. 1979; 167(3):235-41. DOI: 10.1007/BF00267414. View

12.

Holmgren R, Livak K, Morimoto R, Freund R, Meselson M . Studies of cloned sequences from four Drosophila heat shock loci. Cell. 1979; 18(4):1359-70. DOI: 10.1016/0092-8674(79)90246-0. View

13.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

14.

Swanton M, Greslin A, Prescott D . Arrangement of coding and non-coding sequences in the DNA molecules coding for rRNAs in Oxytricha sp. DNA of ciliated protozoa. VII. Chromosoma. 1980; 77(2):203-15. DOI: 10.1007/BF00329545. View

15.

Swanton M, Heumann J, Prescott D . Gene-sized DNA molecules of the macronuclei in three species of hypotrichs: size distributions and absence of nicks. DNA of ciliated protozoa. VIII. Chromosoma. 1980; 77(2):217-27. DOI: 10.1007/BF00329546. View

16.

Loenen W, Brammar W . A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene. 1980; 10(3):249-59. DOI: 10.1016/0378-1119(80)90054-2. View

17.

Messing J, Crea R, Seeburg P . A system for shotgun DNA sequencing. Nucleic Acids Res. 1981; 9(2):309-21. PMC: 326694. DOI: 10.1093/nar/9.2.309. View

18.

Nock A . RNA and macronuclear transcription in the ciliate Stylonychia mytilus. Chromosoma. 1981; 83(2):209-20. DOI: 10.1007/BF00286790. View

19.

Meyerowitz E, Hogness D . Molecular organization of a Drosophila puff site that responds to ecdysone. Cell. 1982; 28(1):165-76. DOI: 10.1016/0092-8674(82)90386-5. View

20.

BOSWELL R, Klobutcher L, Prescott D . Inverted terminal repeats are added to genes during macronuclear development in Oxytricha nova. Proc Natl Acad Sci U S A. 1982; 79(10):3255-9. PMC: 346394. DOI: 10.1073/pnas.79.10.3255. View