Inverted Terminal Repeats Are Added to Genes During Macronuclear Development in Oxytricha Nova

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 May 1

PMID 6808504

Citations 19

Authors

R E BOSWELL

L A Klobutcher

D M Prescott

Affiliations

Soon will be listed here.

Abstract

In the hypotrichous ciliate Oxytricha nova all of the macronuclear DNA is in the form of low molecular weight, gene-sized molecules with an average size of 2,200 base pairs. These molecules are produced during macronuclear development by excision from micronuclear chromosomes. All, or nearly all, of the small macronuclear DNA molecules possess an inverted terminal repeat sequence consisting of 5' C4A4 3' repeats. The hypothesis that this terminal sequence serves as a recognition signal for excision of gene-sized molecules from chromosomes has been tested. A sequence containing the C4A4 repeat has been isolated and used to screen clones of micronuclear DNA for the presence of the repeat sequence. The results show that the intact repeated C4A4 sequence is not present at the ends of macronuclear sequences as they exist in the micronuclear chromosomes. Therefore, the entire terminal repeat is not a recognition sequence for gene excision but must be added to the ends of gene-sized molecules during or after the excision process.

Citing Articles

Tec3, a new developmentally eliminated DNA element in Euplotes crassus.

Jacobs M, Sanchez-Blanco A, Katz L, Klobutcher L Eukaryot Cell. 2003; 2(1):103-14.

PMID: 12582127 PMC: 141165. DOI: 10.1128/EC.2.1.103-114.2003.

Conserved DNA sequences adjacent to chromosome fragmentation and telomere addition sites in Euplotes crassus.

Klobutcher L, Gygax S, Podoloff J, Vermeesch J, Price C, Tebeau C Nucleic Acids Res. 1998; 26(18):4230-40.

PMID: 9722644 PMC: 147820. DOI: 10.1093/nar/26.18.4230.

Volatility of internal eliminated segments in germ line genes of hypotrichous ciliates.

DuBois M, Prescott D Mol Cell Biol. 1997; 17(1):326-37.

PMID: 8972213 PMC: 231757. DOI: 10.1128/MCB.17.1.326.

The DNA of ciliated protozoa.

Prescott D Microbiol Rev. 1994; 58(2):233-67.

PMID: 8078435 PMC: 372963. DOI: 10.1128/mr.58.2.233-267.1994.

Scrambling of the actin I gene in two Oxytricha species.

Dubois M, Prescott D Proc Natl Acad Sci U S A. 1995; 92(9):3888-92.

PMID: 7732002 PMC: 42067. DOI: 10.1073/pnas.92.9.3888.

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