The Radiation Response of V79 and Human Tumour Multicellular Spheroids--cell Survival and Growth Delay Studies

Overview

Journal Br J Cancer

Specialty Oncology

Date 1984 Aug 1

PMID 6087864

Citations 9

Authors

C M West

R R Sandhu

I J Stratford

Affiliations

Soon will be listed here.

Abstract

Chinese hamster cells (V79 379A) cells from a human small cell carcinoma of the lung (ME/MAR) and two xenografted human melanomas (HX117 and HX118) have been grown as multicellular spheroids in vitro. The radiation response of these four cell types has been compared when grown as spheroids (200 or 400 micron in diameter) and as single cells from disaggregated spheroids. The radiation sensitivity of the three human lines irradiated as single cells in air, is similar. In comparison, the V79 cells are more radioresistant. Only the V79 and HX118 cells show a spheroid size dependent radiation response. The radiation response of spheroids has been assayed using both cell survival and growth delay. V79, ME/MAR and HX117 cells demonstrate a good correlation between the two endpoints whereas with HX118 there appears to be greater cell kill for a given level of growth delay. This may be because HX118 is efficient in the repair of potentially lethal damage (PLD). The results support the view that extrinsic factors such as three dimensional contact, hypoxia and repair of PLD can be important and together with the intrinsic cell radiosensitivity will determine the radiation response of tumours.

Citing Articles

Preliminary evaluation of prostate-targeted radiotherapy using (131) I-MIP-1095 in combination with radiosensitising chemotherapeutic drugs.

Tesson M, Rae C, Nixon C, Babich J, Mairs R J Pharm Pharmacol. 2016; 68(7):912-21.

PMID: 27139157 PMC: 5298040. DOI: 10.1111/jphp.12558.

Contribution of HIF-1 and drug penetrance to oxaliplatin resistance in hypoxic colorectal cancer cells.

Roberts D, Williams K, Cowen R, Barathova M, Eustace A, Brittain-Dissont S Br J Cancer. 2009; 101(8):1290-7.

PMID: 19755992 PMC: 2768443. DOI: 10.1038/sj.bjc.6605311.

Growth and radiosensitivity of malignant melanoma multicellular spheroids initiated directly from surgical specimens of tumours in man.

Rofstad E Br J Cancer. 1986; 54(4):569-78.

PMID: 3778801 PMC: 2001490. DOI: 10.1038/bjc.1986.211.

Deriving cell survival curves from the overall responses of irradiated tumours: analysis of published data for tumour spheroids.

Moore J, West C, Hendry J Br J Cancer. 1987; 56(3):309-14.

PMID: 3663479 PMC: 2002203. DOI: 10.1038/bjc.1987.194.

Multicellular spheroids. A review on cellular aggregates in cancer research.

Mueller-Klieser W J Cancer Res Clin Oncol. 1987; 113(2):101-22.

PMID: 3549738 DOI: 10.1007/BF00391431.

References

Durand R, Sutherland R . Effects of intercellular contact on repair of radiation damage. Exp Cell Res. 1972; 71(1):75-80. DOI: 10.1016/0014-4827(72)90265-0. View

Durand R, Sutherland R . Dependence of the radiation response of an in vitro tumor model on cell cycle effects. Cancer Res. 1973; 33(2):213-9. View

Sutherland R, Durand R . Hypoxic cells in an in vitro tumour model. Int J Radiat Biol Relat Stud Phys Chem Med. 1973; 23(3):235-46. DOI: 10.1080/09553007314550261. View

Kopper L, STEEL G . The therapeutic response of three human tumor lines maintained in immune-suppressed mice. Cancer Res. 1975; 35(10):2704-13. View

Dertinger H, Lucke Huhle C . A comparative study of post-irradiation growth kinetics of spheroids and monolayers. Int J Radiat Biol Relat Stud Phys Chem Med. 1975; 28(3):255-65. DOI: 10.1080/09553007514551011. View

Cooke B, Fielden E, Johnson M . Polyfunctional radiosensitizers. I. Effects of a nitroxyl biradical on the survival of mammalian cells in vitro. Radiat Res. 1976; 65(1):152-62. View

COURTENAY V . A soft agar colony assay for Lewis lung tumour and B16 melanoma taken directly from the mouse. Br J Cancer. 1976; 34(1):39-45. PMC: 2025124. DOI: 10.1038/bjc.1976.119. View

Durand R . Cell cycle kinetics in an in vitro tumor model. Cell Tissue Kinet. 1976; 9(5):403-12. DOI: 10.1111/j.1365-2184.1976.tb01290.x. View

Yuhas J, Li A, Martinez A, LADMAN A . A simplified method for production and growth of multicellular tumor spheroids. Cancer Res. 1977; 37(10):3639-43. View

10.

COURTENAY V, Mills J . An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. Br J Cancer. 1978; 37(2):261-8. PMC: 2009601. DOI: 10.1038/bjc.1978.35. View

11.

Carlsson J . Proliferation and viability in cellular spheroids of human origin. Cancer Res. 1978; 38(5):1457-64. View

12.

Millar B, Fielden E, Millar J . Interpretation of survival-curve data for Chinese hamster cells, line V-79 using the multi-target, multi-target with initial slope, and alpha, beta equations. Int J Radiat Biol Relat Stud Phys Chem Med. 1978; 33(6):599-603. DOI: 10.1080/09553007814550521. View

13.

Durand R . Variable radiobiological responses of spheroids. Radiat Res. 1980; 81(1):85-99. View

14.

Brown J, Twentyman P, Zamvil S . Response to the RIF-1 tumor in vitro and in C3H/Km mice to X-radiation (cell survival, regrowth delay, and tumor control), chemotherapeutic agents, and activated macrophages. J Natl Cancer Inst. 1980; 64(3):605-11. View

15.

Twentyman P . Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival. Br J Cancer. 1980; 42(2):297-304. PMC: 2010392. DOI: 10.1038/bjc.1980.230. View

16.

Bailey M, Gazet J, Smith I, STEEL G . Chemotherapy of human breast-carcinoma xenografts. Br J Cancer. 1980; 42(4):530-6. PMC: 2010453. DOI: 10.1038/bjc.1980.276. View

17.

POURREAU-SCHNEIDER N, Malaise E . Relationship between surviving fractions using the colony methods, the LD50, and the growth delay after irradiation of human melanoma cells grown as multicellular spheroids. Radiat Res. 1981; 85(2):321-32. View

18.

Chavaudra N, Guichard M, Malaise E . Hypoxic fraction and repair of potentially lethal radiation damage in two human melanomas transplanted into nude mice. Radiat Res. 1981; 88(1):56-68. View

19.

Weichselbaum R, Schmit A, Little J . Cellular repair factors influencing radiocurability of human malignant tumours. Br J Cancer. 1982; 45(1):10-6. PMC: 2010946. DOI: 10.1038/bjc.1982.2. View

20.

Guichard M, Malaise E . Radiosensitivity of Nall human melanoma transplanted into nude mice: repair, reoxygenation and dose fractionation. Int J Radiat Oncol Biol Phys. 1982; 8(6):1005-10. DOI: 10.1016/0360-3016(82)90169-9. View