Growth and Radiosensitivity of Malignant Melanoma Multicellular Spheroids Initiated Directly from Surgical Specimens of Tumours in Man

Overview

Journal Br J Cancer

Specialty Oncology

Date 1986 Oct 1

PMID 3778801

Citations 1

Authors

E K Rofstad

Affiliations

Soon will be listed here.

Abstract

The growth and radiosensitivity of multicellular spheroids initiated directly from disaggregated surgical specimens of four human malignant melanomas were studied. The spheroids were grown in liquid-overlay culture for up to 6 passages. Cell survival following irradiation was measured by using the Courtenay soft agar colony assay. The four melanomas formed spherical, densely packed spheroids. The volumetric growth rate as well as the plating efficiency in soft agar usually increased with increasing passage number. The radiosensitivity differed significantly among the melanomas. The survival curves for single cells from disaggregated spheroids in the first passage were always similar to those for single cells isolated directly from the surgical specimens. Two of the melanomas showed a significant contact effect as spheroids whereas the other two did not. The spheroids of two of the melanomas showed lower D0 in the third and the sixth passage than in the first passage, whereas the spheroids of the other two melanomas showed similar survival curves in the first and the third passage. There was no clear relationship between the changes in radiosensitivity and the changes in growth rate or plating efficiency. It is concluded that spheroids in the first passage, but not spheroids in later passages, may have the potential to identify differences in clinical radioresponsiveness among tumours.

Citing Articles

Growth and radiation sensitivity of the MLS human ovarian carcinoma cell line grown as multicellular spheroids and xenografted tumours.

Rofstad E, Sutherland R Br J Cancer. 1989; 59(1):28-35.

PMID: 2757922 PMC: 2246982. DOI: 10.1038/bjc.1989.7.

References

Sutherland R, Inch W, McCredie J, Kruuv J . A multi-component radiation survival curve using an in vitro tumour model. Int J Radiat Biol Relat Stud Phys Chem Med. 1970; 18(5):491-5. DOI: 10.1080/09553007014551401. View

Rofstad E . Radiation biology of malignant melanoma. Acta Radiol Oncol. 1986; 25(1):1-10. DOI: 10.3109/02841868609136368. View

Sutherland R, Durand R . Hypoxic cells in an in vitro tumour model. Int J Radiat Biol Relat Stud Phys Chem Med. 1973; 23(3):235-46. DOI: 10.1080/09553007314550261. View

Sutherland R, Durand R . Radiation response of multicell spheroids--an in vitro tumour model. Curr Top Radiat Res Q. 1976; 11(1):87-139. View

Durand R, Sutherland R . Repair and reoxygenation following irradiation of an in vitro tumor model. Int J Radiat Oncol Biol Phys. 1976; 1(11-12):1119-24. DOI: 10.1016/0360-3016(76)90084-5. View

COURTENAY V, Mills J . An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. Br J Cancer. 1978; 37(2):261-8. PMC: 2009601. DOI: 10.1038/bjc.1978.35. View

Yuhas J, Li A . Growth fraction as the major determinant of multicellular tumor spheroid growth rates. Cancer Res. 1978; 38(6):1528-32. View

Habermalz H . Irradiation of malignant melanoma: experience in the past and present. Int J Radiat Oncol Biol Phys. 1981; 7(1):131-3. DOI: 10.1016/0360-3016(81)90072-9. View

Trott K, von Lieven H, Kummermehr J, Skopal D, Lukacs S, Braun-Falco O . The radiosensitivity of malignant melanomas part II: clinical studies. Int J Radiat Oncol Biol Phys. 1981; 7(1):15-20. DOI: 10.1016/0360-3016(81)90054-7. View

10.

Lobo P, LIEBNER E, Chao J, Kanji A . Radiotherapy in the management of malignant melanoma. Int J Radiat Oncol Biol Phys. 1981; 7(1):21-6. DOI: 10.1016/0360-3016(81)90055-9. View

11.

Fertil B, Malaise E . Inherent cellular radiosensitivity as a basic concept for human tumor radiotherapy. Int J Radiat Oncol Biol Phys. 1981; 7(5):621-9. DOI: 10.1016/0360-3016(81)90377-1. View

12.

Rofstad E . Radiation response of the cells of a human malignant melanoma xenograft. Effect of hypoxic cell radiosensitizers. Radiat Res. 1981; 87(3):670-83. View

13.

Harwood A, Cummings B . Radiotherapy for malignant melanoma: a re-appraisal. Cancer Treat Rev. 1981; 8(4):271-82. DOI: 10.1016/s0305-7372(81)80011-4. View

14.

Jones A, Stratford I, Wilson P, Peckham M . In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids. Br J Cancer. 1982; 46(6):870-9. PMC: 2011214. DOI: 10.1038/bjc.1982.296. View

15.

Darling J, Oktar N, Thomas D . Multicellular tumour spheroids derived from human brain tumours. Cell Biol Int Rep. 1983; 7(1):23-30. DOI: 10.1016/0309-1651(83)90101-7. View

16.

Twentyman P . Exclusion of host cells during spheroid formation from disaggregated solid tumours. Br J Cancer. 1983; 47(4):541-3. PMC: 2011335. DOI: 10.1038/bjc.1983.85. View

17.

Carlsson J, Nilsson K, Westermark B, Ponten J, Sundstrom C, Larsson E . Formation and growth of multicellular spheroids of human origin. Int J Cancer. 1983; 31(5):523-33. DOI: 10.1002/ijc.2910310502. View

18.

Wibe E, Berg J, Tveit K, Nesland J, LUNDE S . Multicellular spheroids grown directly from human tumour material. Int J Cancer. 1984; 34(1):21-6. DOI: 10.1002/ijc.2910340105. View

19.

West C, Sandhu R, Stratford I . The radiation response of V79 and human tumour multicellular spheroids--cell survival and growth delay studies. Br J Cancer. 1984; 50(2):143-51. PMC: 1976873. DOI: 10.1038/bjc.1984.156. View

20.

Deacon J, Peckham M, STEEL G . The radioresponsiveness of human tumours and the initial slope of the cell survival curve. Radiother Oncol. 1984; 2(4):317-23. DOI: 10.1016/s0167-8140(84)80074-2. View