A Comparative Study of Post-irradiation Growth Kinetics of Spheroids and Monolayers

Overview

Journal Int J Radiat Biol Relat Stud Phys Chem Med

Specialties Biochemistry
Biophysics
Nuclear Medicine
Radiology

Date 1975 Sep 1

PMID 1081512

Citations 11

Authors

H Dertinger

C Lucke Huhle

Affiliations

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Abstract

Post-irradiation growth kinetics of gamma-irradiated spheroid and monolayer cells in exponential growth phase was investigated by means of dose-response curves based on cell counts after specified time intervals following irradiation. A mathematical model of cell-growth after irradiation was fitted to these curves. The model parameters (related to division delay and growth of non-surviving cellsy obtained from this analysis consistently indicate increasing resistance to sub-lethal damage of cells cultured as multicellular spheroids under conditions of increasing three-dimensional contact. In contrast, no indication of an increased radiation-resistance was found with cells cultured on a substratum under a variety of conditions.

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Increased radioresistance of cells in cultured multicell spheroids. II. Kinetic and cytogenetic studies.

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