Sporulation in Bacillus Subtilis. Correlation of Biochemical Events with Morphological Changes in Asporogenous Mutants

Overview

Journal Biochem J

Specialty Biochemistry

Date 1970 Jul 1

PMID 4991477

Citations 36

Authors

W M Waites

D Kay

I W Dawes

D A Wood

S C Warren

J MANDELSTAM

Affiliations

Soon will be listed here.

Abstract

A comparison was made of morphological changes and successive, mainly biochemical, marker events for sporulation in 14 asporogenous mutants. The morphological and biochemical sequences are linked so that arrested development in one is accompanied by corresponding effects in the other. Thus mutants that fail to produce both protease and antibiotic do not progress beyond stage 0, formation of alkaline phosphatase appears to be associated with the transition from stage II to stage III and glucose dehydrogenase with that from stage III to stage IV. Stage II mutants may produce ;pygmy' cells or other bizarre cell-division forms. The biochemical sequence is dependent in the sense that if the occurrence of any one event is blocked that of all the succeeding events is also blocked. This has implications for biochemical models that have been proposed to explain the temporal sequence observed in spore development.

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References

RYTER A, SZULMAJSTER J . [Effect of chloramphenicol on the sporogenesis of B. subtilis]. Ann Inst Pasteur (Paris). 1965; 108(5):640-51. View

Rouyard J, IONESCO H, Schaeffer P . [Genetic classification of certain sporulation mutants of Bacillus subtilis, Marburg]. Ann Inst Pasteur (Paris). 1967; 113(5):675-83. View

Kornberg A, Spudich J, Nelson D, Deutscher M . Origin of proteins in sporulation. Annu Rev Biochem. 1968; 37:51-78. DOI: 10.1146/annurev.bi.37.070168.000411. View

MANDELSTAM J, Waites W . Sporulation in Bacillus subtilis. The role of exoprotease. Biochem J. 1968; 109(5):793-801. PMC: 1187030. DOI: 10.1042/bj1090793. View

Warren S . Sporulation in Bacillus subtilis. Biochemical changes. Biochem J. 1968; 109(5):811-8. PMC: 1187032. DOI: 10.1042/bj1090811. View

Kay D, Warren S . Sporulation in Bacillus subtilis. Morphological changes. Biochem J. 1968; 109(5):819-24. PMC: 1187033. DOI: 10.1042/bj1090819. View

Schaeffer P . Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969; 33(1):48-71. PMC: 378312. DOI: 10.1128/br.33.1.48-71.1969. View

Dawes I, Kay D, MANDELSTAM J . Sporulation in Bacillus subtilis. Establishment of a time scale for the morphological events. J Gen Microbiol. 1969; 56(2):171-9. DOI: 10.1099/00221287-56-2-171. View

STERLINI J, MANDELSTAM J . Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem J. 1969; 113(1):29-37. PMC: 1184601. DOI: 10.1042/bj1130029. View

10.

Losick R, Sonenshein A . Change in the template specificity of RNA polymerase during sporulation of Bacillus subtilis. Nature. 1969; 224(5214):35-7. DOI: 10.1038/224035a0. View

11.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

12.

STANIER R . Enzymatic adaptation in bacteria. Annu Rev Microbiol. 1951; 5:35-56. DOI: 10.1146/annurev.mi.05.100151.000343. View

13.

Donnellan Jr J, Nags E, LEVINSON H . CHEMICALLY DEFINED, SYNTHETIC MEDIA FOR SPORULATION AND FOR GERMINATION AND GROWTH OF BACILLUS SUBTILIS. J Bacteriol. 1964; 87:332-6. PMC: 277012. DOI: 10.1128/jb.87.2.332-336.1964. View

14.

SZULMAJSTER J . [BIOCHEMISTRY OF SPOROGENESIS IN B. SUBTILIS]. Bull Soc Chim Biol (Paris). 1964; 46:443-81. View

15.

Young I . CHARACTERISTICS OF AN ABORTIVELY DISPORIC VARIANT OF BACILLUS CEREUS. J Bacteriol. 1964; 88:242-54. PMC: 277283. DOI: 10.1128/jb.88.1.242-254.1964. View

16.

Takahashi I . LOCALIZATION OF SPORE MARKERS ON THE CHROMOSOME OF BACILLUS SUBTILIS. J Bacteriol. 1965; 89:1065-7. PMC: 277596. DOI: 10.1128/jb.89.4.1065-1067.1965. View

17.

Burton K . A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956; 62(2):315-23. PMC: 1215910. DOI: 10.1042/bj0620315. View