Alpha 2.0
Login Register
» Articles » PMID: 5001733

Sporulation in Bacillus Subtilis. The Appearance of Sulpholactic Acid As a Marker Event for Sporulation

Overview
Journal Biochem J
Specialty Biochemistry
Date 1971 Jul 1
PMID 5001733
Citations 8
Authors
D A Wood
Affiliations
Soon will be listed here.
Abstract

1. The synthesis of sulpholactic acid in sporulating cultures of Bacillus subtilis was studied. 2. Sulpholactic acid was first detected about 4h after the initiation of sporulation and 1h before refractility. The rate of synthesis paralleled that of the other events of sporulation examined. 3. Sulpholactic acid accounted for 1.7% of the material of the spore. 4. Because the addition of chloramphenicol in the earlier stages of sporulation inhibited formation of the compound, it is likely that the enzymes concerned are synthesized de novo during sporulation. 5. In asporogenous mutants only those blocked at a late stage and showing partial refractility were able to produce sulpholactic acid. This correlation makes sulpholactic acid a useful marker event in sporulation.

Citing Articles

Anaerobic Degradation of the Plant Sugar Sulfoquinovose Concomitant With HS Production: K-12 and sp. Strain DF1 as Co-culture Model.

Burrichter A, Denger K, Franchini P, Huhn T, Muller N, Spiteller D Front Microbiol. 2018; 9:2792.

PMID: 30546350 PMC: 6278857. DOI: 10.3389/fmicb.2018.02792.

Glycolytic breakdown of sulfoquinovose in bacteria: a missing link in the sulfur cycle.

Roy A, Hewlins M, Ellis A, Harwood J, White G Appl Environ Microbiol. 2003; 69(11):6434-41.

PMID: 14602597 PMC: 262304. DOI: 10.1128/AEM.69.11.6434-6441.2003.

Time of enterotoxin formation and release during sporulation of Clostridium perfringens type A.

Duncan C J Bacteriol. 1973; 113(2):932-6.

PMID: 4347930 PMC: 285311. DOI: 10.1128/jb.113.2.932-936.1973.

Sporulation and enterotoxin production by mutants of Clostridium perfringens.

Duncan C, Strong D, Sebald M J Bacteriol. 1972; 110(1):378-91.

PMID: 4336110 PMC: 247421. DOI: 10.1128/jb.110.1.378-391.1972.

Sporulation in Bacillus subtilis. Properties and time of synthesis of alkali-soluble protein of the spore coat.

Wood D Biochem J. 1972; 130(2):505-14.

PMID: 4198082 PMC: 1174430. DOI: 10.1042/bj1300505.

References
1.
MANDELSTAM J, Waites W . Sporulation in Bacillus subtilis. The role of exoprotease. Biochem J. 1968; 109(5):793-801. PMC: 1187030. DOI: 10.1042/bj1090793. View
2.
Kay D, Warren S . Sporulation in Bacillus subtilis. Morphological changes. Biochem J. 1968; 109(5):819-24. PMC: 1187033. DOI: 10.1042/bj1090819. View
3.
Schaeffer P . Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969; 33(1):48-71. PMC: 378312. DOI: 10.1128/br.33.1.48-71.1969. View
4.
Bonsen P, Spudich J, Nelson D, Kornberg A . Biochemical studies of bacterial sporulation and germination. XII. A sulfonic acid as a major sulfur compound of Bacillus subtilis spores. J Bacteriol. 1969; 98(1):62-8. PMC: 249904. DOI: 10.1128/jb.98.1.62-68.1969. View
5.
Dawes I, Kay D, MANDELSTAM J . Sporulation in Bacillus subtilis. Establishment of a time scale for the morphological events. J Gen Microbiol. 1969; 56(2):171-9. DOI: 10.1099/00221287-56-2-171. View