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Abnormal Septation and Inhibition of Sporulation by Accumulation of L- -glycerophosphate in Bacillus Subtilis Mutants

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1973 Feb 1
PMID 4632310
Citations 19
Authors
Y K O
E B Freese
E Freese
Affiliations
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Abstract

Accumulation of l-alpha-glycerophosphate, in cells of Bacillus subtilis mutants lacking the nicotinamide adenine dinucleotide-independent glycerophosphate dehydrogenase activity, suppresses both growth and sporulation. After growth has stopped, the cells slowly develop one and later more asymmetric septa that are thicker than normal prespore septa and apparently contain too much cell wall material to allow further membrane development into forespores or spores. l-Malate prevents accumulation of glycerophosphate and restores sporulation of the mutant. Glucose or gluconate cannot resotre sporulation, because they still effect glycerophosphate accumulation via de novo synthesis. If that accumulation is blocked in a double mutant, which is unable to make glycerophosphate from or to metabolize it into Embden-Meyerhof compounds, then nonsuppressing amounts of glucose or gluconate can restore sporulation.

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References
1.
RYTER A, Schaeffer P, IONESCO H . [Cytologic classification, by their blockage stage, of sporulation mutants of Bacillus subtilis Marburg]. Ann Inst Pasteur (Paris). 1966; 110(3):305-15. View
2.
Fortnagel P, Freese E . Analysis of sporulation mutants. II. Mutants blocked in the citric acid cycle. J Bacteriol. 1968; 95(4):1431-8. PMC: 315104. DOI: 10.1128/jb.95.4.1431-1438.1968. View
3.
Schaeffer P . Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969; 33(1):48-71. PMC: 378312. DOI: 10.1128/br.33.1.48-71.1969. View
4.
Yamamoto T, Balassa G . Biochemical genetics of bacterial sporulation. II. Membrane development suring sporulation of B. subtilis and its mutants. Mol Gen Genet. 1969; 106(1):1-13. DOI: 10.1007/BF00332816. View
5.
Berman M, ZWAIG N, Lin E . Suppression of a pleiotropic mutant affecting glycerol dissimilation. Biochem Biophys Res Commun. 1970; 38(2):272-8. DOI: 10.1016/0006-291x(70)90708-4. View