Rapid Gentamicin Bioassay Using a Multiple-antibiotic-resistant Strain of Klebsiella Pneumoniae

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1973 Nov 1

PMID 4598849

Citations 22

Authors

M E Lund

D J Blazevic

J M Matsen

Affiliations

Soon will be listed here.

Abstract

A rapid bioassay for gentamicin levels in serum was developed by using a strain of Klebsiella pneumoniae that has multiple resistance to antibiotics. Assays were comparable when performed on either small or large petri plates, and results were available in 2 to 4 h. Studies showed an overall recovery of 97.6% for gentamicin alone or 104.5% in the presence of commonly used antibiotics. The procedure can be performed without the need to inactivate other antibiotics that may be present in the serum sample.

Citing Articles

Biographical feature: John Matsen, M.D.

Carroll K J Clin Microbiol. 2014; 52(8):2750-2.

PMID: 24920778 PMC: 4136141. DOI: 10.1128/JCM.01542-14.

Use of Carbapenems against clinical, nontyphoid Salmonella isolates: results from in vitro and in vivo animal studies.

Tang H, Chen C, Zhang C, Cheng K, Chiang S, Chiu Y Antimicrob Agents Chemother. 2012; 56(6):2916-22.

PMID: 22470122 PMC: 3370812. DOI: 10.1128/AAC.00110-12.

Peritoneal fluid concentrations of gentamicin in patients with spontaneous bacterial peritonitis.

Richey G, Schleupner C Antimicrob Agents Chemother. 1981; 19(2):312-5.

PMID: 7347563 PMC: 181417. DOI: 10.1128/AAC.19.2.312.

Latex agglutination inhibition card test for gentamicin assay: clinical evaluation and comparison with radioimmunoassay and bioassay.

Standiford H, Bernstein D, Nipper H, Caplan E, Tatem B, Hall J Antimicrob Agents Chemother. 1981; 19(4):620-4.

PMID: 7247384 PMC: 181489. DOI: 10.1128/AAC.19.4.620.

Evaluation of the macro-vue latex agglutination test for quantitation of gentamicin in human serum.

Johnson J, Crawford S, Jorgensen J J Clin Microbiol. 1982; 16(2):299-302.

PMID: 7119102 PMC: 272349. DOI: 10.1128/jcm.16.2.299-302.1982.

References

Perkins R, SASLAW S . Experiences with cephalothin. Ann Intern Med. 1966; 64(1):13-24. DOI: 10.7326/0003-4819-64-1-13. View

BAUER A, KIRBY W, SHERRIS J, Turck M . Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4):493-6. View

Bennett J, BRODIE J, BENNER E, KIRBY W . Simplified, accurate method for antibiotic assay of clinical specimens. Appl Microbiol. 1966; 14(2):170-7. PMC: 546645. DOI: 10.1128/am.14.2.170-177.1966. View

Faine S, Knight D . Rapid microbiological assay of antibiotic in blood and other body fluids. Lancet. 1968; 2(7564):375-8. DOI: 10.1016/s0140-6736(68)90593-x. View

Stroy S . Modified microbiological assay for rapid estimation of antibiotic concentrations in human sera. Appl Microbiol. 1969; 18(1):31-4. PMC: 377879. DOI: 10.1128/am.18.1.31-34.1969. View

Kunin C . Binding of antibiotics to tissue homogenates. J Infect Dis. 1970; 121(1):55-64. DOI: 10.1093/infdis/121.1.55. View

Noone P, Pattison J, Samson D . Simple, rapid method for assay of aminoglycoside antibiotics. Lancet. 1971; 2(7714):16-9. DOI: 10.1016/s0140-6736(71)90007-9. View

Stroy S, Preston D . Specific assay of aminoglycosidic- or polymyxin-type antibiotics present in human sera in combination with cephalosporins. Appl Microbiol. 1971; 21(6):1002-6. PMC: 377332. DOI: 10.1128/am.21.6.1002-1006.1971. View

Sabath L, CASEY J, Ruch P, Stumpf L, FINLAND M . Rapid microassay of gentamicin, kanamycin, neomycin, streptomycin, and vancomycin in serum or plasma. J Lab Clin Med. 1971; 78(3):457-63. View

10.

Sabath L, CASEY J, Ruch P, Stumpf L, FINLAND M . Rapid microassay for circulating nephrotoxic antibiotics. Antimicrob Agents Chemother (Bethesda). 1970; 10:83-90. DOI: 10.1128/AAC.10.1.83. View

11.

Medeiros A, OBrien T, Wacker W, Yulug N . Effect of salt concentration on the apparent in-vitro susceptibility of Pseudomonas and other gram-negative bacilli to gentamicin. J Infect Dis. 1971; 124 Suppl:S59-64. DOI: 10.1093/infdis/124.supplement_1.s59. View

12.

Winters R, Litwack K, HEWITT W . Relation between dose and levels of gentamicin in blood. J Infect Dis. 1971; 124 Suppl:S90-5. DOI: 10.1093/infdis/124.supplement_1.s90. View

13.

Davis W, Stout T . Disc plate method of microbiological antibiotic assay. I. Factors influencing variability and error. Appl Microbiol. 1971; 22(4):659-65. PMC: 376382. DOI: 10.1128/am.22.4.659-665.1971. View

14.

Smith D, Van Otto B, Smith A . A rapid chemical assay for gentamicin. N Engl J Med. 1972; 286(11):583-6. DOI: 10.1056/NEJM197203162861106. View

15.

Lewis J, Nelson J, ELDER H . Radioimmunoassay of an antibiotic: gentamicin. Nat New Biol. 1972; 239(94):214-6. DOI: 10.1038/newbio239214a0. View

16.

Noone P, Pattison J, Slack R . Rapid assay of gentamicin. Lancet. 1972; 2(7788):1194-5. DOI: 10.1016/s0140-6736(72)92612-8. View

17.

Reeves D . Assay of gentamicin. Lancet. 1972; 2(7791):1369-70. DOI: 10.1016/s0140-6736(72)92816-4. View

18.

Noone P, Pattison J, Slack R . Assay of gentamicin. Lancet. 1973; 1(7793):49-50. DOI: 10.1016/s0140-6736(73)91275-0. View

19.

Yourassowsky E, DeBroe M, Wieme R . Effect of heparin on gentamicin concentration in blood. Clin Chim Acta. 1972; 42(1):189-91. DOI: 10.1016/0009-8981(72)90394-4. View

20.

Warren E, Snyder R, Washington JA I . Four-hour microbiological assay of gentamicin in serum. Antimicrob Agents Chemother. 1972; 1(1):46-8. PMC: 444164. DOI: 10.1128/AAC.1.1.46. View