Use of Immunoperoxidase for the Rapid Identification of Human Myxoviruses and Paramyxoviruses in Tissue Culture

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1974 Jul 1

PMID 4367457

Citations 8

Authors

D R Benjamin

C G RAY

Affiliations

Soon will be listed here.

Abstract

The immunoperoxidase method, using commercially available antisera, was compared with standard virological methods for the identification and typing of 77 isolates of human myxoviruses and paramyxoviruses. Results of typing using neutralization tests and the immunoperoxidase technique were identical for 76 of the 77 isolates. With the immunoperoxidase method there was one false negative reaction, but no false positive reactions. Cross-reactivity between influenza A (soluble) and A(2)/HK antisera with influenza A isolates was noted, but did not interfere with the interpretation of results. It is concluded that the immunoperoxidase method is ideally suited for the rapid identification and typing of common human respiratory viruses on a routine basis. It offers a number of decided advantages over both immunofluorescence and standard virological methods.

Citing Articles

[Procedures of viral identification in respiratory infections].

Aspa J, Cardenoso L Arch Bronconeumol. 1995; 31(9):470-80.

PMID: 8520820 PMC: 7131635. DOI: 10.1016/s0300-2896(15)30868-1.

Development of an immunofluorescence focus assay for Ebola virus.

Truant A, Regnery R, Kiley M J Clin Microbiol. 1983; 18(2):416-9.

PMID: 6352735 PMC: 270815. DOI: 10.1128/jcm.18.2.416-419.1983.

Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method.

Benjamin D Appl Microbiol. 1974; 28(4):568-71.

PMID: 4370826 PMC: 186773. DOI: 10.1128/am.28.4.568-571.1974.

Rapid and accurate viral diagnosis.

Landry M, Mayo D, Hsiung G Pharmacol Ther. 1989; 40(2):287-328.

PMID: 2499895 PMC: 7133644. DOI: 10.1016/0163-7258(89)90098-3.

Assessment of virus infectivity by the immunofluorescent and immunoperoxidase techniques.

Hahon N, Simpson J, ECKERT H J Clin Microbiol. 1975; 1(3):324-9.

PMID: 1100661 PMC: 275074. DOI: 10.1128/jcm.1.3.324-329.1975.

References

GRAY K, Macfarlane D, SOMMERVILLE R . Direct immunofluorescent identification of respiratory syncytial virus in throat swabs from children with respiratory illness. Lancet. 1968; 1(7540):446-8. DOI: 10.1016/s0140-6736(68)92779-7. View

Gardner P, MCQUILLIN J . Application of immunofluorescent antibody technique in rapid diagnosis of respiratory syncytial virus infection. Br Med J. 1968; 3(5614):340-3. PMC: 1991592. DOI: 10.1136/bmj.3.5614.340. View

Stevens T, WATKINS H . Rapid identification of viruses by indirect immunofluorescence: standardization and use of antiserum pool to nine respiratory viruses. Appl Microbiol. 1969; 17(3):384-93. PMC: 377698. DOI: 10.1128/am.17.3.384-393.1969. View

Wicker R, Avrameas S . Localization of virus antigens by enzyme-labelled antibodies. J Gen Virol. 1969; 4(4):465-71. DOI: 10.1099/0022-1317-4-4-465. View

DAlessio D, Williams S, DICK E . Rapid detection and identification of respiratory viruses by direct immunofluorescence. Appl Microbiol. 1970; 20(2):233-9. PMC: 376907. DOI: 10.1128/am.20.2.233-239.1970. View

URQUHART G, Martin K . Respiratory syncytial virus tissue culture immunofluorescence as a laboratory aid. Arch Gesamte Virusforsch. 1970; 32(4):365-72. DOI: 10.1007/BF01250064. View

Ubertini T, Wilkie B, Noronha F . Use of horseradish peroxidase-labeled antibody for light and electron microscope localization of reovirus antigen. Appl Microbiol. 1971; 21(3):534-8. PMC: 377217. DOI: 10.1128/am.21.3.534-538.1971. View

Herd S, MacWilliam K . Preparation of antiviral sera for immunofluorescence on infected tissue culture. J Clin Pathol. 1971; 24(4):304-7. PMC: 476979. DOI: 10.1136/jcp.24.4.304. View

CRADOCK-WATSON J, MCQUILLIN J, Gardner P . Rapid diagnosis of respiratory syncytial virus infection in children by the immunofluorescent technique. J Clin Pathol. 1971; 24(4):308-12. PMC: 476980. DOI: 10.1136/jcp.24.4.308. View

10.

Marks M, Nagahama H, Eller J . Parainfluenza virus immunofluorescence. In vitro and clinical application of the direct method. Pediatrics. 1971; 48(1):73-8. View

11.

Wicker R . Comparison of immunofluorescent and immunoenzymatic techniques applied to the study of viral antigens. Ann N Y Acad Sci. 1971; 177:490-500. DOI: 10.1111/j.1749-6632.1971.tb35079.x. View

12.

Shabo A, Petricciani J, KIRSCHSTEIN R . Immunoperoxidase localization of herpes zoster virus and simian virus 40 in cell culture. Appl Microbiol. 1972; 23(5):1001-9. PMC: 380490. DOI: 10.1128/am.23.5.1001-1009.1972. View

13.

Avrameas S, Ternynck T . Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971; 8(12):1175-9. DOI: 10.1016/0019-2791(71)90395-8. View

14.

Dobardzic R, Boudreault A, PAVILANIS V . [Identification of influenza virus with the aid of immunoperoxidase]. Can J Microbiol. 1973; 19(1):146-9. View

15.

Clyne D, Norris S, Modesto R, Pesce A, POLLAK V . Antibody enzyme conjugates: the preparation of intermolecular conjugates of horseradish peroxidase and antibody and their use in immunohistology of renal cortex. J Histochem Cytochem. 1973; 21(3):233-40. DOI: 10.1177/21.3.233. View

16.

Rahman A . Early detection of influenza virus in cell culture by means of immunofluorescence. J Clin Pathol. 1973; 26(7):503-5. PMC: 477804. DOI: 10.1136/jcp.26.7.503. View

17.

Herrmann J, Morse S . Coupling of peroxidase to poliovirus antibody: characteristics of the conjugates and their use in virus detection. Infect Immun. 1973; 8(4):645-9. PMC: 422904. DOI: 10.1128/iai.8.4.645-649.1973. View

18.

KAPLOW L . SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE. Blood. 1965; 26:215-9. View