Early Detection of Influenza Virus in Cell Culture by Means of Immunofluorescence

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1973 Jul 1

PMID 4199361

Citations 2

Authors

A F Rahman

Affiliations

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Abstract

An indirect fluorescent antibody technique using human serum for the rapid detection of influenza A virus in monkey kidney cell cultures is described. Influenza A2 virus was isolated from 38 of 88 nose/throat swabs received from patients with suspected influenza during the winter of 1971-72.Thirty-two strains isolated in monkey kidney cell cultures were identified by immunofluorescence on the day haemadsorption was observed, 22 of them within a week of receipt of the specimen. Human antiserum was found to be very satisfactory for this purpose.

Citing Articles

Use of immunoperoxidase for the rapid identification of human myxoviruses and paramyxoviruses in tissue culture.

Benjamin D, RAY C Appl Microbiol. 1974; 28(1):47-51.

PMID: 4367457 PMC: 186585. DOI: 10.1128/am.28.1.47-51.1974.

Subtype-specific identification of influenza virus in cell cultures with FITC labelled egg yolk antibodies.

Doller P, Doller G, GERTH H Med Microbiol Immunol. 1987; 176(1):27-35.

PMID: 3547056 DOI: 10.1007/BF00189406.

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