Rapid Identification of Viruses by Indirect Immunofluorescence: Standardization and Use of Antiserum Pool to Nine Respiratory Viruses

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1969 Mar 1

PMID 4305395

Citations 8

Authors

T D Stevens

H M WATKINS

Affiliations

Soon will be listed here.

Abstract

The indirect fluorescent antibody test employing treated and standardized antisera and conjugated antiglobulin has been used successfully, in conjunction with a technique for growing and staining virus cell systems in situ on microscope slides, in the identification of nine respiratory viruses. By using pooled antisera in a single test, the presence or absence of these viruses was determined in 18 to 45 hr after inoculation of slide microtissue culture.

Citing Articles

Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques.

Minnich L, RAY C J Clin Microbiol. 1980; 12(3):391-4.

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Rapid identification of viruses by indirect immunofluorescence: isolation and identification of adenovirus types 4 and 7 and coxsackievirus type A21 in microcultures.

Stevens T, WATKINS H Appl Microbiol. 1971; 22(5):784-96.

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Use of immunoperoxidase for the rapid identification of human myxoviruses and paramyxoviruses in tissue culture.

Benjamin D, RAY C Appl Microbiol. 1974; 28(1):47-51.

PMID: 4367457 PMC: 186585. DOI: 10.1128/am.28.1.47-51.1974.

An intersecting anti-serum pool system for the immunofluorescent identification of respiratory viruses.

MClure A, Macfarlane D, SOMMERVILLE R Arch Gesamte Virusforsch. 1972; 37(1):6-11.

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Antibodies in urine of chimpanzees with chronic adenoviral viruria.

Ashner L, Asher D, Shah K, Amyx H, Gibbs Jr C, Gajdusek D Infect Immun. 1978; 21(2):458-61.

PMID: 211084 PMC: 422018. DOI: 10.1128/iai.21.2.458-461.1978.

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