Assessment of Virus Infectivity by the Immunofluorescent and Immunoperoxidase Techniques

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1975 Mar 1

PMID 1100661

Citations 1

Authors

N Hahon

J Simpson

H L ECKERT

Affiliations

Soon will be listed here.

Abstract

The immunofluorescent and immunoperoxidase cell-counting techniques were comparable in precision and reproducibility for the quantitative assessment of influenza virus infectivity. The dose-response function was linear with each procedure, and comparable results were obtained for estimating neutralizing antibodies in antiviral serum.

Citing Articles

Interferon assessment by the immunofluorescent, immunoperoxidase, and hemadsorption cell-counting techniques.

Hahon N, Booth J, ECKERT H Arch Virol. 1975; 48(3):239-43.

PMID: 170887 DOI: 10.1007/BF01317966.

References

Nakane P, Pierce Jr G . Enzyme-labeled antibodies: preparation and application for the localization of antigens. J Histochem Cytochem. 1966; 14(12):929-31. DOI: 10.1177/14.12.929. View

Hahon N . Multiple infection of cell monolayers by virus mixtures. Appl Microbiol. 1967; 15(2):458-9. PMC: 546927. DOI: 10.1128/am.15.2.458-459.1967. View

Wong S, KILBOURNE E . Changing viral susceptibility of a human cell line in continuous cultivation. I. Production of infective virus in a variant of the Chang conjunctival cell following infection with swine or N-WS influenza viruses. J Exp Med. 1961; 113:95-110. PMC: 2137333. DOI: 10.1084/jem.113.1.95. View

Rodriguez J, Deinhardt F . Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology. 1960; 12:316-7. DOI: 10.1016/0042-6822(60)90205-1. View

Shabo A, Petricciani J, KIRSCHSTEIN R . Immunoperoxidase localization of herpes zoster virus and simian virus 40 in cell culture. Appl Microbiol. 1972; 23(5):1001-9. PMC: 380490. DOI: 10.1128/am.23.5.1001-1009.1972. View

Dubois-Dalcq M, Barbosa L . Immunoperoxidase stain of measles antigen in tissue culture. J Virol. 1973; 12(4):909-18. PMC: 356710. DOI: 10.1128/JVI.12.4.909-918.1973. View

Herrmann J, Morse S . Coupling of peroxidase to poliovirus antibody: characteristics of the conjugates and their use in virus detection. Infect Immun. 1973; 8(4):645-9. PMC: 422904. DOI: 10.1128/iai.8.4.645-649.1973. View

Avrameas S . Immunoenzyme techniques: enzymes as markers for the localization of antigens and antibodies. Int Rev Cytol. 1970; 27:349-85. DOI: 10.1016/s0074-7696(08)61250-4. View

Kurstak E, Belloncik S, Onji P, Montplaisir S, Martineau B . [Localization by immunoperoxidase of cytomegalovirus antigens in human fibroblast cell cultures. Light and electron microscopy]. Arch Gesamte Virusforsch. 1972; 38(1):67-76. View

10.

Siverd N, Sharon N . Immunohistochemical method for detection of vaccinia virus. Proc Soc Exp Biol Med. 1969; 131(3):939-41. DOI: 10.3181/00379727-131-34014. View

11.

Benjamin D, RAY C . Use of immunoperoxidase for the rapid identification of human myxoviruses and paramyxoviruses in tissue culture. Appl Microbiol. 1974; 28(1):47-51. PMC: 186585. DOI: 10.1128/am.28.1.47-51.1974. View

12.

Herrmann J, Morse S, Collins M . Comparison of techniques and immunoreagents used for indirect immunofluorescence and immunoperoxidase identification of enteroviruses. Infect Immun. 1974; 10(1):220-6. PMC: 414981. DOI: 10.1128/iai.10.1.220-226.1974. View

13.

Wicker R . Comparison of immunofluorescent and immunoenzymatic techniques applied to the study of viral antigens. Ann N Y Acad Sci. 1971; 177:490-500. DOI: 10.1111/j.1749-6632.1971.tb35079.x. View

14.

Hahon N, Booth J, ECKERT H . Cell attachment and penetration by influenza virus. Infect Immun. 1973; 7(3):341-51. PMC: 422681. DOI: 10.1128/iai.7.3.341-351.1973. View

15.

LEVADITI J, ATANASIU P, Gamet A, GUILLON J . [Diagnosis of rabies by the immunofluorescence and immuno-peroxidase methods]. Bull Soc Pathol Exot Filiales. 1973; 66(1):12-20. View

16.

Avrameas S, Uriel J . [Method of antigen and antibody labelling with enzymes and its immunodiffusion application]. C R Acad Hebd Seances Acad Sci D. 1966; 262(24):2543-5. View

17.

Hahon N, COOKE K . Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus. J Virol. 1967; 1(2):317-26. PMC: 375230. DOI: 10.1128/JVI.1.2.317-326.1967. View

18.

Oxford J, Schild G . Immunofluorescent studies on the inhibition of influenza A and B viruses in mammalian cell cultures by amines and ammonium compounds. J Gen Virol. 1968; 2(3):377-84. DOI: 10.1099/0022-1317-2-3-377. View

19.

Kozikowski E, Hahon N . Quantitative assay of interferon by the immunofluorescent cell-counting technique. J Gen Virol. 1969; 4(3):441-3. DOI: 10.1099/0022-1317-4-3-441. View

20.

CARTER G . THE RAPID DETECTION, TITRATION, AND DIFFERENTIATION OF VARIOLA AND VACCINIA VIRUSES BY A FLUORESCENT ANTIBODY-COVERSLIP CELL MONOLAYER SYSTEM. Virology. 1965; 25:659-62. DOI: 10.1016/0042-6822(65)90094-2. View