Synergy Between Subpopulations of Mouse Spleen Cells in the in Vitro Generation of Cell-mediated Cytotoxicity: Evidence for the Involvement of a Non-T Cell

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1974 Dec 1

PMID 4279272

Citations 21

Authors

R J Hodes

B S Handwerger

W D TERRY

Affiliations

Soon will be listed here.

Abstract

Two subpopulations separated from normal spleen have been shown to synergize as responding cells in the in vitro induction of specific cell-mediated cytotoxicity during the mixed lymphocyte culture (MLC). The synergizing populations are a nylon wool column-adherent and a nylon wool column-nonadherent fraction, enriched for B lymphocytes and T lymphocytes, respectively. When a mixture of these fractions is used as the responding cell population in MLC, greater cytotoxicity is generated than would be expected from the sum of activities generated in the two subpopulations sensitized separately. The synergy appears to occur at the sensitization rather than the effector phase. The synergizing cell which is contained in the nylon-adherent subpopulation is distinct from the cytotoxic effector T lymphocyte, is resistant to lysis by rabbit antimouse brain serum, and is unresponsive to phytohemagglutinin; its synergizing function could not be replaced by either plastic-adherent spleen cells or peritoneal exudate cells. These results suggest a role of a non-T-cell nonmacrophage population in the generation of cytotoxic activity.

Citing Articles

Cerebral protection in homozygous null ICAM-1 mice after middle cerebral artery occlusion. Role of neutrophil adhesion in the pathogenesis of stroke.

Connolly Jr E, Winfree C, Springer T, Naka Y, Liao H, Yan S J Clin Invest. 1996; 97(1):209-16.

PMID: 8550836 PMC: 507081. DOI: 10.1172/JCI118392.

Mitogenic activity of Sindbis virus and its isolated glycoproteins.

McSharry J Infect Immun. 1982; 38(3):1242-8.

PMID: 6984018 PMC: 347881. DOI: 10.1128/iai.38.3.1242-1248.1982.

Mitogenicity of Naegleria fowleri extract for murine T lymphocytes.

Ferrante A, Smyth C Immunology. 1984; 51(3):461-8.

PMID: 6607875 PMC: 1454447.

Chlorozotocin, an anti-tumour agent lacking bone marrow toxicity at therapeutic doses: effects on lymphocyte subpopulations in mice.

Fisher R, Mandell G, Bostick F, McMenamin M, Anderson T Clin Exp Immunol. 1980; 39(2):416-25.

PMID: 6446427 PMC: 1538078.

Sendai virus glycoproteins are T cell-dependent B cell mitogens.

Kizaka S, McSharry J Infect Immun. 1983; 40(2):592-600.

PMID: 6302000 PMC: 264895. DOI: 10.1128/iai.40.2.592-600.1983.

References

Mishell R, Dutton R . Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967; 126(3):423-42. PMC: 2138377. DOI: 10.1084/jem.126.3.423. View

Mosier D . A requirement for two cell types for antibody formation in vitro. Science. 1967; 158(3808):1573-5. DOI: 10.1126/science.158.3808.1573. View

Miller J, Mitchell G . Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968; 128(4):801-20. PMC: 2138540. DOI: 10.1084/jem.128.4.801. View

Cline M, Lehrer R . Phagocytosis by human monocytes. Blood. 1968; 32(3):423-35. View

Hayry P, Defendi V . Mixed lymphocyte cultures produce effector cells: model in vitro for allograft rejection. Science. 1970; 168(3927):133-5. DOI: 10.1126/science.168.3927.133. View

Hodes R, Svedmyr E . Specific cytotoxicity of H-2-incompatible mouse lymphocytes following mixed culture in vitro. Transplantation. 1970; 9(5):470-7. DOI: 10.1097/00007890-197005000-00006. View

Lonai P, Feldman M . Cooperation of lymphoid cells in an in vitro graft reaction system. II. The "bone marrow-derived" cell. Transplantation. 1971; 11(5):446-56. DOI: 10.1097/00007890-197105000-00003. View

Jones A . Effect of column purification on response of lymphocytes in mixed leucocyte cultures (MLC) and to blastogenic media deprived from MLCS. Clin Exp Immunol. 1971; 8(6):927-39. PMC: 1713047. View

Golub E . Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells. Cell Immunol. 1971; 2(4):353-61. DOI: 10.1016/0008-8749(71)90070-0. View

10.

Svedmyr E, Hodes R . On the specificity of cell-mediated cytotoxicity in vitro. Cell Immunol. 1970; 1(6):644-54. DOI: 10.1016/0008-8749(70)90029-8. View

11.

Claman H, Chaperon E . Immunologic complementation between thymus and marrow cells--a model for the two-cell theory of immunocompetence. Transplant Rev. 1969; 1:92-113. DOI: 10.1111/j.1600-065x.1969.tb00137.x. View

12.

CANTOR H, Asofsky R . Synergy among lymphoid cells mediating the graft-versus-host response. 3. Evidence for interaction between two types of thymus-derived cells. J Exp Med. 1972; 135(4):764-79. PMC: 2139160. DOI: 10.1084/jem.135.4.764. View

13.

Wagner H, Feldmann M, BOYLE W, Schrader J . Cell-mediated immune response in vitro. 3. The requirement for macrophages in cytotoxic reactions against cell-bound and subcellular alloantigens. J Exp Med. 1972; 136(2):331-43. PMC: 2139211. DOI: 10.1084/jem.136.2.331. View

14.

Tigelaar R, Asofsky R . Synergy among lymphoid cells mediating the graft-versus-host response. V. Derivation by migration in lethally irradiated recipients of two interacting subpopulations of thymus-derived cells from normal spleen. J Exp Med. 1973; 137(2):239-53. PMC: 2139477. DOI: 10.1084/jem.137.2.239. View

15.

Feldman M, Cohen I, Wekerle H . T-cell mediated immunity in vitro: an analysis of antigen recognition and target cell lysis. Transplant Rev. 1972; 12:57-90. DOI: 10.1111/j.1600-065x.1972.tb00053.x. View

16.

Cohen L, Howe M . Synergism between subpopulations of thymus-derived cells mediating the proliferative and effector phases of the mixed lymphocyte reaction. Proc Natl Acad Sci U S A. 1973; 70(9):2707-10. PMC: 427088. DOI: 10.1073/pnas.70.9.2707. View

17.

Wagner H . Synergy during in vitro cytotoxic allograft responses. I. Evidence for cell interaction between thymocytes and peripheral T cells. J Exp Med. 1973; 138(6):1379-97. PMC: 2139468. DOI: 10.1084/jem.138.6.1379. View

18.

Zembala M, Ptak W, Hanczakowska M . Macrophage and lymphocyte co-operation in target cell destruction in vitro. Clin Exp Immunol. 1973; 15(3):461-6. PMC: 1553897. View

19.

Julius M, Simpson E, Herzenberg L . A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973; 3(10):645-9. DOI: 10.1002/eji.1830031011. View

20.

Hayry P, Andersson L . T cells in mixed-lymphocyte-culture-induced cytolysis (MLC-CML). Transplant Proc. 1973; 5(4):1697-703. View