Interstrain Conservation of the Murine GAT-specific Antibody V Kappa Repertoire As Analyzed at the Germline Gene Level

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1983 Jan 1

PMID 6139278

Citations 9

Authors

C Schiff

S Corbet

M Milili

M Fougereau

Affiliations

Soon will be listed here.

Abstract

A cDNA library was constructed in pBR322 from mRNA encoding an anti-GAT (Glu60 Ala30 Tyr10) monoclonal antibody kappa chain. Two cDNA clones were extensively characterized. One, L XI 62, was derived from an aberrant V kappa-J kappa rearrangement which resulted in a frame-shift at position 96, leading to a stop codon at the very beginning of the constant region. The second, L XIX 27, 1150 bp long, was unequivocally assigned to a GAT-specific kappa chain, by comparison of its nucleotide sequence with the previously determined NH2-terminal amino acid sequence of the isolated kappa chain. A specific probe, containing the leader and most of the V kappa gene-encoded region, was prepared from this clone and hybridized to EcoRI and BamHI restriction fragments of liver (unrearranged) DNA extracted from the BALB/c, DBA/2 and C57BL/6 mouse strains. Under stringent conditions, similar patterns were observed for all three strains, and consisted of a small number of bands (3-5). Under nonstringent conditions, patterns were again very similar when the different strains were compared, although 15-20 bands could be identified. These observations support the hypothesis that the GAT-specific kappa chains found in antibodies expressing the public CGAT idiotypes are encoded by a very small number of germline genes. This V kappa repertoire seems extremely conserved between the three strains that were analyzed, an observation which correlates with the interstrain conservation of these public idiotypic specificities.

Citing Articles

The idiotypic network and the internal image: possible regulation of a germ-line network by paucigene encoded Ab2 (anti-idiotypic) antibodies in the GAT system.

Ollier P, Somme G, Theze J, Fougereau M EMBO J. 1985; 4(13B):3681-8.

PMID: 3937730 PMC: 554718. DOI: 10.1002/j.1460-2075.1985.tb04135.x.

Preferential rearrangement of the immunoglobulin kappa chain joining region J kappa 1 and J kappa 2 segments in mouse spleen DNA.

Nishi M, Kataoka T, Honjo T Proc Natl Acad Sci U S A. 1985; 82(19):6399-403.

PMID: 3931074 PMC: 390723. DOI: 10.1073/pnas.82.19.6399.

Gene repertoire of the anti-poly(Glu60Ala30Tyr10) (GAT) immune response: comparison of VH, V kappa, and D regions used by anti-GAT antibodies and monoclonal antibodies produced after anti-idiotypic immunization.

Roth C, Somme G, Fougereau M, Theze J Proc Natl Acad Sci U S A. 1985; 82(14):4788-92.

PMID: 3927291 PMC: 390990. DOI: 10.1073/pnas.82.14.4788.

Genetic elements used for a murine lupus anti-DNA autoantibody are closely related to those for antibodies to exogenous antigens.

Kofler R, Noonan D, Levy D, Wilson M, Moller N, Dixon F J Exp Med. 1985; 161(4):805-15.

PMID: 3920343 PMC: 2189057. DOI: 10.1084/jem.161.4.805.

Specificities and V genes encoding monoclonal autoantibodies from viable motheaten mice.

Painter C, Monestier M, Chew A, Kasturi K, Bailey C, Scott V J Exp Med. 1988; 167(3):1137-53.

PMID: 3351435 PMC: 2188884. DOI: 10.1084/jem.167.3.1137.

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