Uridylation Regulates MRNA Decay Directionality in Fission Yeast

Overview

Journal Nat Commun

Specialty Biology

Date 2024 Sep 27

PMID 39333464

Authors

Maciej Grochowski

Lidia Lipinska-Zubrycka

StJohn Townsend

Anna Golisz-Mocydlarz

Monika Zakrzewska-Placzek

Grzegorz Brzyzek

Borna Jurkovic

Szymon Swiezewski

Markus Ralser

Michal Malecki

Affiliations

Soon will be listed here.

Abstract

Cytoplasmic mRNA decay is effected by exonucleolytic degradation in either the 5' to 3' or 3' to 5' direction. Pervasive terminal uridylation is implicated in mRNA degradation, however, its functional relevance for bulk mRNA turnover remains poorly understood. In this study, we employ genome-wide 3'-RACE (gw3'-RACE) in the model system fission yeast to elucidate the role of uridylation in mRNA turnover. We observe widespread uridylation of shortened poly(A) tails, promoting efficient 5' to 3' mRNA decay and ensuring timely and controlled mRNA degradation. Inhibition of this uridylation process leads to excessive deadenylation and enhanced 3' to 5' mRNA decay accompanied by oligouridylation. Strikingly we found that uridylation of poly(A) tails and oligouridylation of non-polyadenylated substrates are catalysed by different terminal uridyltransferases Cid1 and Cid16 respectively. Our study sheds new light on the intricate regulatory mechanisms underlying bulk mRNA turnover, demonstrating the role of uridylation in modulating mRNA decay pathways.

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