Bifunctional Sesquiterpene/Diterpene Synthase Agr2 from Gives Rise to the Novel Diterpene Cyclocybene

Overview

Journal ACS Chem Biol

Publisher American Chemical Society

Specialties Biochemistry
Biology

Date 2024 Sep 18

PMID 39293797

Authors

Nikolas Hoberg

Karen Harms

Frank Surup

Martin Ruhl

Affiliations

Soon will be listed here.

Abstract

is a model mushroom belonging to the fungal phylum Basidiomycota. Among others, is known for its diverse terpenome, containing various volatile and nonvolatile terpenes and terpenoids. Here, we deepen the knowledge on their biosynthetic pathways by studying the terpene synthase Agr2 in detail. In contrast to previous studies, the heterologous production of Agr2 in the agaric host revealed the production of two terpenes, one of which was the already known sesquiterpene viridiflorene. The other one was a so far unknown diterpene that had to be isolated and purified by means of preparative RP-HPLC for structure elucidation. 1D- and 2D-NMR experiments revealed the compound as the novel diterpene cyclocybene, pointing to the bifunctionality of Agr2 to produce both a sesquiterpene and a diterpene.

Citing Articles

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PMID: 39883255 PMC: 11740858. DOI: 10.1007/s44307-024-00056-2.

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