DNA-mediated Transformation of the Basidiomycete Coprinus Cinereus

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1987 Apr 1

PMID 3595558

Citations 83

Authors

D M Binninger

C Skrzynia

P J Pukkila

L A Casselton

Affiliations

Soon will be listed here.

Abstract

We have developed a simple and efficient transformation system for the agaric fungus, Coprinus cinereus. Protoplasts were prepared from asexual spores that harbor one or two mutations in the structural gene for tryptophan synthetase. The protoplasts can be stably transformed using the cloned Coprinus gene at a frequency of 1 in 10(4) viable protoplasts. A variety of molecular events accompanies the formation of stable transformants, including insertion of the transforming DNA at the homologous locus. The transforming DNA is stable through cell division, mating, fruiting body formation, and meiosis.

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