Expression of the RAD1 and RAD3 Genes of Saccharomyces Cerevisiae is Not Affected by DNA Damage or During the Cell Division Cycle

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1985 Jan 1

PMID 3923299

Citations 9

Authors

M L Nagpal

D R Higgins

S Prakash

Affiliations

Soon will be listed here.

Abstract

The RAD1 and RAD3 genes of Saccharomyces cerevisiae are required for excision repair of UV damaged DNA. In addition, the RAD3 gene is essential since rad3 deletions are recessive lethals. We have examined the induction of the RAD1 and RAD3 genes by DNA damage and during the cell division cycle. We have made fusions of the RAD1 and RAD3 genes with the Escherichia coli lacZ gene encoding beta-galactosidase. Beta-galactosidase activity was measured in a Rad+ yeast strain containing the RAD1-lacZ or the RAD3-lacZ fusion, either in a multicopy replicating plasmid or as a single copy integrant resulting from transformation with an integrating plasmid which transforms yeast by homologous recombination in the yeast genome. No induction of beta-galactosidase activity occurred after ultraviolet light (UV) or 4-nitroquinoline-1-oxide (NQO) treatment. Haploid cells of mating type a were synchronized by treatment with alpha factor and beta-galactosidase activity was determined during different cell cycle stages. No change in beta-galactosidase activity was observed in the strain containing the RAD1-lacZ or the RAD3-lacZ fusion integrated in the yeast genome.

Citing Articles

Promoter elements of the PHR1 gene of Saccharomyces cerevisiae and their roles in the response to DNA damage.

Sancar G, Ferris R, Smith F, Vandeberg B Nucleic Acids Res. 1995; 23(21):4320-8.

PMID: 7501452 PMC: 307386. DOI: 10.1093/nar/23.21.4320.

Nucleotide sequence of the RAD10 gene of Saccharomyces cerevisiae.

Reynolds P, Prakash L, Dumais D, Perozzi G, Prakash S EMBO J. 1985; 4(13A):3549-52.

PMID: 3912171 PMC: 554695. DOI: 10.1002/j.1460-2075.1985.tb04115.x.

Nucleotide sequence and functional analysis of the RAD1 gene of Saccharomyces cerevisiae.

Reynolds P, Prakash L, Prakash S Mol Cell Biol. 1987; 7(3):1012-20.

PMID: 3550428 PMC: 365171. DOI: 10.1128/mcb.7.3.1012-1020.1987.

Regulation of the RAD6 gene of Saccharomyces cerevisiae in the mitotic cell cycle and in meiosis.

Kupiec M, Simchen G Mol Gen Genet. 1986; 203(3):538-43.

PMID: 3528754 DOI: 10.1007/BF00422083.

DNA repair genes of Saccharomyces cerevisiae: complementing rad4 and rev2 mutations by plasmids which cannot be propagated in Escherichia coli.

Siede W, Eckardt-Schupp F Curr Genet. 1986; 11(3):205-10.

PMID: 3329049 DOI: 10.1007/BF00420608.

References

Scherer S, Davis R . Replacement of chromosome segments with altered DNA sequences constructed in vitro. Proc Natl Acad Sci U S A. 1979; 76(10):4951-5. PMC: 413056. DOI: 10.1073/pnas.76.10.4951. View

Kenyon C, Walker G . Expression of the E. coli uvrA gene is inducible. Nature. 1981; 289(5800):808-10. DOI: 10.1038/289808a0. View

Jachymczyk W, VON Borstel R, Mowat M, Hastings P . Repair of interstrand cross-links in DNA of Saccharomyces cerevisiae requires two systems for DNA repair: the RAD3 system and the RAD51 system. Mol Gen Genet. 1981; 182(2):196-205. DOI: 10.1007/BF00269658. View

Easton A, Kushner S . Transcription of the uvrD gene of Escherichia coli is controlled by the lexA repressor and by attenuation. Nucleic Acids Res. 1983; 11(24):8625-40. PMC: 326612. DOI: 10.1093/nar/11.24.8625. View

Naumovski L, Friedberg E . A DNA repair gene required for the incision of damaged DNA is essential for viability in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983; 80(15):4818-21. PMC: 384136. DOI: 10.1073/pnas.80.15.4818. View

Backendorf C, Brandsma J, Kartasova T, Van de Putte P . In vivo regulation of the uvrA gene: role of the "-10" and "-35" promoter regions. Nucleic Acids Res. 1983; 11(17):5795-810. PMC: 326318. DOI: 10.1093/nar/11.17.5795. View

Rose M, Botstein D . Construction and use of gene fusions to lacZ (beta-galactosidase) that are expressed in yeast. Methods Enzymol. 1983; 101:167-80. DOI: 10.1016/0076-6879(83)01012-5. View

Higgins D, Prakash S, Reynolds P, Polakowska R, Weber S, Prakash L . Isolation and characterization of the RAD3 gene of Saccharomyces cerevisiae and inviability of rad3 deletion mutants. Proc Natl Acad Sci U S A. 1983; 80(18):5680-4. PMC: 384322. DOI: 10.1073/pnas.80.18.5680. View

Higgins D, Prakash S, Reynolds P, Prakash L . Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae. Gene. 1983; 26(2-3):119-26. DOI: 10.1016/0378-1119(83)90181-6. View

10.

Ruby S, Szostak J . Specific Saccharomyces cerevisiae genes are expressed in response to DNA-damaging agents. Mol Cell Biol. 1985; 5(1):75-84. PMC: 366680. DOI: 10.1128/mcb.5.1.75-84.1985. View

11.

Henriques J, Chanet R, MOUSTACCHI E . The fate of 8-methoxypsoralen photoinduced crosslinks in nuclear and mitochondrial yeast DNA: comparison of wild-type and repair-deficient strains. Proc Natl Acad Sci U S A. 1982; 79(6):1722-6. PMC: 346052. DOI: 10.1073/pnas.79.6.1722. View

12.

Williamson D . Replication of the nuclear genome in yeast does not require concomitant protein synthesis. Biochem Biophys Res Commun. 1973; 52(3):731-40. DOI: 10.1016/0006-291x(73)90998-4. View

13.

Peterson T, Prakash L, Prakash S, Osley M, Reed S . Regulation of CDC9, the Saccharomyces cerevisiae gene that encodes DNA ligase. Mol Cell Biol. 1985; 5(1):226-35. PMC: 366697. DOI: 10.1128/mcb.5.1.226-235.1985. View

14.

Wilcox D, Prakash L . Incision and postincision steps of pyrimidine dimer removal in excision-defective mutants of Saccharomyces cerevisiae. J Bacteriol. 1981; 148(2):618-23. PMC: 216247. DOI: 10.1128/jb.148.2.618-623.1981. View

15.

SIEGEL E . The Escherichia coli uvrD gene is inducible by DNA damage. Mol Gen Genet. 1983; 191(3):397-400. DOI: 10.1007/BF00425753. View

16.

Sancar G, Sancar A, Little J, RUPP W . The uvrB gene of Escherichia coli has both lexA-repressed and lexA-independent promoters. Cell. 1982; 28(3):523-30. DOI: 10.1016/0092-8674(82)90207-0. View

17.

Schendel P, Fogliano M, Strausbaugh L . Regulation of the Escherichia coli K-12 uvrB operon. J Bacteriol. 1982; 150(2):676-85. PMC: 216416. DOI: 10.1128/jb.150.2.676-685.1982. View

18.

Casadaban M, Chou J, Cohen S . In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol. 1980; 143(2):971-80. PMC: 294402. DOI: 10.1128/jb.143.2.971-980.1980. View

19.

Nakayama K, Irino N, Nakayama H . recA+ gene-dependent regulation of a uvrD::lacZ fusion in Escherichia coli K12. Mol Gen Genet. 1983; 192(3):391-4. DOI: 10.1007/BF00392180. View

20.

Miller R, Prakash L, Prakash S . Genetic control of excision of Saccharomyces cerevisiae interstrand DNA cross-links induced by psoralen plus near-UV light. Mol Cell Biol. 1982; 2(8):939-48. PMC: 369882. DOI: 10.1128/mcb.2.8.939-948.1982. View