Replacement of Chromosome Segments with Altered DNA Sequences Constructed in Vitro

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Oct 1

PMID 388424

Citations 368

Authors

S Scherer

R W Davis

Affiliations

Soon will be listed here.

Abstract

We have developed a method that may be of general application for the stable introduction of foreign sequences or deletions, constructed in vitro, into the chromosomes of Saccharomyces cerevisiae. No vector sequences are present in the final strains. Ability to transform cells with DNA, availability of a single selective marker, and integration of the transforming DNA by homologous recombination into the chromosomes are the requirements of the system. Any isolated gene can be deleted or altered and then be used to replace the wild-type chromosomal copy. An internal deletion mutant of the his3 gene and a transposition of a galactose-inducible region into chromosome XV have been generated by using the ura3 gene as the selective marker.

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References

Morse M, LEDERBERG E, LEDERBERG J . Transductional Heterogenotes in Escherichia Coli. Genetics. 1956; 41(5):758-79. PMC: 1209815. DOI: 10.1093/genetics/41.5.758. View

Kelley W, Chalmers K, Murray N . Isolation and characterization of a lambdapolA transducing phage. Proc Natl Acad Sci U S A. 1977; 74(12):5632-6. PMC: 431839. DOI: 10.1073/pnas.74.12.5632. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Hicks J, Hinnen A, Fink G . Properties of yeast transformation. Cold Spring Harb Symp Quant Biol. 1979; 43 Pt 2:1305-13. DOI: 10.1101/sqb.1979.043.01.149. View

Bach M, Lacroute F, Botstein D . Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli. Proc Natl Acad Sci U S A. 1979; 76(1):386-90. PMC: 382944. DOI: 10.1073/pnas.76.1.386. View

Struhl K, Stinchcomb D, Scherer S, Davis R . High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci U S A. 1979; 76(3):1035-9. PMC: 383183. DOI: 10.1073/pnas.76.3.1035. View

St John T, Davis R . Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridization. Cell. 1979; 16(2):443-52. DOI: 10.1016/0092-8674(79)90020-5. View

Hinnen A, Hicks J, Fink G . Transformation of yeast. Proc Natl Acad Sci U S A. 1978; 75(4):1929-33. PMC: 392455. DOI: 10.1073/pnas.75.4.1929. View

Cameron J, Philippsen P, Davis R . Analysis of chromosomal integration and deletions of yeast plasmids. Nucleic Acids Res. 1977; 4(5):1429-48. PMC: 343766. DOI: 10.1093/nar/4.5.1429. View

10.

Ratzkin B, Carbon J . Functional expression of cloned yeast DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1977; 74(2):487-91. PMC: 392314. DOI: 10.1073/pnas.74.2.487. View

11.

McDonell M, Simon M, Studier F . Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977; 110(1):119-46. DOI: 10.1016/s0022-2836(77)80102-2. View

12.

Bach M, Lacroute F . Direct selective techniques for the isolation of pyrimidine auxotrophs in yeast. Mol Gen Genet. 1972; 115(2):126-30. DOI: 10.1007/BF00277292. View

13.

Struhl K, Cameron J, Davis R . Functional genetic expression of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1976; 73(5):1471-5. PMC: 430318. DOI: 10.1073/pnas.73.5.1471. View

14.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

15.

Snow R . An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'. Nature. 1966; 211(5045):206-7. DOI: 10.1038/211206a0. View