Promoter Elements of the PHR1 Gene of Saccharomyces Cerevisiae and Their Roles in the Response to DNA Damage

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1995 Nov 11

PMID 7501452

Citations 6

Authors

G B Sancar

R Ferris

F W Smith

B Vandeberg

Affiliations

Soon will be listed here.

Abstract

The PHR1 gene of Saccharomyces cerevisiae encodes the apoenzyme for the DNA repair enzyme photolyase. PHR1 transcription is induced in response to 254 nm radiation and a variety of chemical damaging agents. We report here the identification of promoter elements required for PHR1 expression. Transcription is regulated primarily through three sequence elements clustered within a 120 bp region immediately upstream of the translational start site. A 20 bp interrupted palindrome comprises UASPHR1 and is responsible for 80-90% of basal and induced expression. UASPHR1 alone can activate transcription of a CYC1 minimal promoter but does not confer damage responsiveness. In the intact PHR1 promoter UAS function is dependent upon an upstream essential sequence (UES). URSPHR1 contains a binding site for the damage-responsive repressor Prp; consistent with this role, deletion or specific mutations of the URS increase basal level expression and decrease the induction ratio. Deletion of URSPHR1 also eliminates the requirement for UESPHR1 for promoter activation, indicating that the UES attenuates Prp-mediated repression. Sequences within UASPHR1 are similar to regulatory sequences found upstream of both damage responsive and nonresponsive genes involved in DNA repair and metabolism.

Citing Articles

The histone H3K36 demethylase Rph1/KDM4 regulates the expression of the photoreactivation gene PHR1.

Liang C, Hsu P, Chou D, Pan C, Wang L, Huang W Nucleic Acids Res. 2011; 39(10):4151-65.

PMID: 21296759 PMC: 3105397. DOI: 10.1093/nar/gkr040.

Rdp1, a novel zinc finger protein, regulates the DNA damage response of rhp51(+) from Schizosaccharomyces pombe.

Shim Y, Jang Y, Lim M, Lee J, Seong R, Hong S Mol Cell Biol. 2000; 20(23):8958-68.

PMID: 11073995 PMC: 86550. DOI: 10.1128/MCB.20.23.8958-8968.2000.

Regulatory networks revealed by transcriptional profiling of damaged Saccharomyces cerevisiae cells: Rpn4 links base excision repair with proteasomes.

Jelinsky S, Estep P, Church G, Samson L Mol Cell Biol. 2000; 20(21):8157-67.

PMID: 11027285 PMC: 86425. DOI: 10.1128/MCB.20.21.8157-8167.2000.

RPH1 and GIS1 are damage-responsive repressors of PHR1.

Jang Y, Wang L, Sancar G Mol Cell Biol. 1999; 19(11):7630-8.

PMID: 10523651 PMC: 84794. DOI: 10.1128/MCB.19.11.7630.

Role of UME6 in transcriptional regulation of a DNA repair gene in Saccharomyces cerevisiae.

Sweet D, Jang Y, Sancar G Mol Cell Biol. 1997; 17(11):6223-35.

PMID: 9343383 PMC: 232473. DOI: 10.1128/MCB.17.11.6223.

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