Transcription of the UvrD Gene of Escherichia Coli is Controlled by the LexA Repressor and by Attenuation

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1983 Dec 20

PMID 6324092

Citations 23

Authors

A M Easton

S R Kushner

Affiliations

Soon will be listed here.

Abstract

The nucleotide sequence of the control region and the presumptive N-terminal portion of the uvrD gene of Escherichia coli K-12 has been determined. The 1190 base pairs of DNA examined include the likely coding sequence for the first 258 amino acids of the uvrD protein. The transcription promoter for the uvrD gene was identified upstream of the protein coding region. Synthesis of messenger RNA in vitro from this promoter was inhibited by purified lexA protein. The lexA protein was found to bind downstream from the promoter at a sequence, CTGTATATATACCCAG, which is homologous to other known lexA protein binding sites. In the absence of the lexA protein, approximately half of the messages initiated in vitro at the uvrD promoter terminate after about 60 nucleotides at a sequence which resembles a rho-independent terminator. These results indicate that the uvrD gene is induced during the SOS response, and that the expression of the gene may also be regulated by transcription attenuation.

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