B Cell Receptor Transgenic Mice As Tools to Study Memory B Cells

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Jul 17

PMID 39017890

Authors

Isaak Quast

Affiliations

Soon will be listed here.

Abstract

B cell receptor (BCR) transgenic mice allow the control of the initial target (antigen) specificity of naïve B cells and to investigate their properties following activation. Here, I describe how BCR transgenic B cells can be used in combination with adoptive cell transfer and immunization models to study memory B cell formation and reactivation.

References

Mesin L, Ersching J, Victora G . Germinal Center B Cell Dynamics. Immunity. 2016; 45(3):471-482. PMC: 5123673. DOI: 10.1016/j.immuni.2016.09.001. View

Jones D, Wilmore J, Allman D . Cellular Dynamics of Memory B Cell Populations: IgM+ and IgG+ Memory B Cells Persist Indefinitely as Quiescent Cells. J Immunol. 2015; 195(10):4753-9. PMC: 4637268. DOI: 10.4049/jimmunol.1501365. View

Akkaya M, Kwak K, Pierce S . B cell memory: building two walls of protection against pathogens. Nat Rev Immunol. 2019; 20(4):229-238. PMC: 7223087. DOI: 10.1038/s41577-019-0244-2. View

Quast I, Tarlinton D . B cell memory: understanding COVID-19. Immunity. 2021; 54(2):205-210. PMC: 7826135. DOI: 10.1016/j.immuni.2021.01.014. View

Pape K, Taylor J, Maul R, Gearhart P, Jenkins M . Different B cell populations mediate early and late memory during an endogenous immune response. Science. 2011; 331(6021):1203-7. PMC: 3993090. DOI: 10.1126/science.1201730. View

Pape K, Maul R, Dileepan T, Paustian A, Gearhart P, Jenkins M . Naive B Cells with High-Avidity Germline-Encoded Antigen Receptors Produce Persistent IgM and Transient IgG Memory B Cells. Immunity. 2018; 48(6):1135-1143.e4. PMC: 6052797. DOI: 10.1016/j.immuni.2018.04.019. View

Zuccarino-Catania G, Sadanand S, Weisel F, Tomayko M, Meng H, Kleinstein S . CD80 and PD-L2 define functionally distinct memory B cell subsets that are independent of antibody isotype. Nat Immunol. 2014; 15(7):631-7. PMC: 4105703. DOI: 10.1038/ni.2914. View

Tarlinton D, Good-Jacobson K . Diversity among memory B cells: origin, consequences, and utility. Science. 2013; 341(6151):1205-11. DOI: 10.1126/science.1241146. View

Weisel N, Joachim S, Smita S, Callahan D, Elsner R, Conter L . Surface phenotypes of naive and memory B cells in mouse and human tissues. Nat Immunol. 2021; 23(1):135-145. PMC: 8712407. DOI: 10.1038/s41590-021-01078-x. View

10.

Weisel F, Zuccarino-Catania G, Chikina M, Shlomchik M . A Temporal Switch in the Germinal Center Determines Differential Output of Memory B and Plasma Cells. Immunity. 2016; 44(1):116-130. PMC: 4724390. DOI: 10.1016/j.immuni.2015.12.004. View

11.

Shinnakasu R, Inoue T, Kometani K, Moriyama S, Adachi Y, Nakayama M . Regulated selection of germinal-center cells into the memory B cell compartment. Nat Immunol. 2016; 17(7):861-9. DOI: 10.1038/ni.3460. View

12.

Mesin L, Schiepers A, Ersching J, Barbulescu A, Cavazzoni C, Angelini A . Restricted Clonality and Limited Germinal Center Reentry Characterize Memory B Cell Reactivation by Boosting. Cell. 2019; 180(1):92-106.e11. PMC: 6958527. DOI: 10.1016/j.cell.2019.11.032. View

13.

Roederer M, Nussenzweig M . Role of antigen receptor affinity in T cell-independent antibody responses in vivo. Nat Immunol. 2002; 3(4):399-406. DOI: 10.1038/ni776. View

14.

Quast I, Dvorscek A, Pattaroni C, Steiner T, McKenzie C, Pitt C . Interleukin-21, acting beyond the immunological synapse, independently controls T follicular helper and germinal center B cells. Immunity. 2022; 55(8):1414-1430.e5. DOI: 10.1016/j.immuni.2022.06.020. View

15.

Phan T, Amesbury M, Gardam S, Crosbie J, Hasbold J, Hodgkin P . B cell receptor-independent stimuli trigger immunoglobulin (Ig) class switch recombination and production of IgG autoantibodies by anergic self-reactive B cells. J Exp Med. 2003; 197(7):845-60. PMC: 2193892. DOI: 10.1084/jem.20022144. View

16.

Brink R, Paus D, Bourne K, Hermes J, Gardam S, Phan T . The SW(HEL) system for high-resolution analysis of in vivo antigen-specific T-dependent B cell responses. Methods Mol Biol. 2015; 1291:103-23. DOI: 10.1007/978-1-4939-2498-1_9. View

17.

Ridderstad A, Tarlinton D . Kinetics of establishing the memory B cell population as revealed by CD38 expression. J Immunol. 1998; 160(10):4688-95. View

18.

Turner J, Marthi M, Benet Z, Grigorova I . Transiently antigen-primed B cells return to naive-like state in absence of T-cell help. Nat Commun. 2017; 8:15072. PMC: 5413946. DOI: 10.1038/ncomms15072. View

19.

Zotos D, Quast I, Li-Wai-Suen C, McKenzie C, Robinson M, Kan A . The concerted change in the distribution of cell cycle phases and zone composition in germinal centers is regulated by IL-21. Nat Commun. 2021; 12(1):7160. PMC: 8660905. DOI: 10.1038/s41467-021-27477-0. View